Reagents: Sol.A = water 30mL + 95%EthOL 70mL + HCl 0.6mL + orcein 1 g Sol.B = HCl 0.3mL + absolute EthOL 97mL Sol.C = Giemsa solution (0.05% eosin + 0.8% azur 5) 0.6mL + water 100mL Sol.D = 0.001% eosin Y in 95%EthOL Method: sections → 70%EthOL → A x ½ to 1 hour → rinse → 95% EthOL a few dips → 100% EthOL til decolorized to pale brwon → B till almost completely decolorized → wash → C x 2 to 12 hours → blot dry → D till dehydrate → clear → mount Recommended for skin section. Ref.: Peter Gray pp 424 Method 21.412 Pinkus 1944 There you have it! René J.
--- On Fri, 6/1/12, Chiriboga, Luis <luis.chirib...@nyumc.org> wrote: From: Chiriboga, Luis <luis.chirib...@nyumc.org> Subject: [Histonet] Special Stain query To: "'histonet@lists.utsouthwestern.edu'" <histonet@lists.utsouthwestern.edu> Date: Friday, June 1, 2012, 2:17 PM Does anyone have a method/protocol for Pinkus’acid orcein-giemsa stain? Thanks and good weekend to all Luis Chiriboga Experimental Pathology Core Laboratory New York University School of Medicine _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
