Was your leica set that cold? Maybe you could try warming the slide for a couple seconds before putting in alcohol. As far a charged slides I am amazed with the slides Dako sells for their IHC.
Sent from my iPhone On Jun 16, 2012, at 12:01 PM, Bernadette del Rosario <badzros...@yahoo.com> wrote: > Hello histonetters!!!Im presently using the Tissue Tek Cryo3 Frozen section > Machine...But i dont understand why i got problems sections falling off > slides.I used different types of slides non adhesive,adhesive(poly l > lysine,thermoshandon,starfrost sakura)..Regular procedure : Sections taken > from 2-4 microns,transfered to 95% alcohol fixative, wolfsgang fixative > etc..then after distilled water wash out...sections started to peel off...My > machine setting- chamber is -30 C;cryochamber is -39 C..example of tissue- > fibroma and salivary gland... > I used Leica cryostat previously but i never got these problems...Even i used > non adhesive but sections still on the slides..Can someone advised > please.Maybe youre using the same tissue tek cryo 3... > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
