Just remember - No formalin, no need for retrieval. In my experience that is the hardest step on tissue sections. It also shortens the procedure a bunch too. Claire
________________________________ From: histonet-boun...@lists.utsouthwestern.edu on behalf of Emily Sours Sent: Sun 6/17/2012 2:34 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Frozen Sections Falling Off Slides Wow you can run frozen sections through those machines? That's awesome. I figured it wouldn't be gentle enough to process them. I'm a fan of Fisher Scientific superfrost plus slides. Fisher claims the slides are electrostatically charged to keep the sections from falling off. Whether that's a bunch of hooha or it's actually true, our sections don't fall off, even after in situs. I believe most scientific suppliers sell their own brand of superfrost plus slides if you don't want to buy from Fisher. Emily "You see a peanut, day's off to a good start; you witness some soil it's a jamboree for Vince Noir." --Howard Moon, in "Charlie", The Mighty Boosh On Sat, Jun 16, 2012 at 12:12 PM, Kim Donadio <one_angel_sec...@yahoo.com>wrote: > Was your leica set that cold? Maybe you could try warming the slide for a > couple seconds before putting in alcohol. As far a charged slides I am > amazed with the slides Dako sells for their IHC. > > Sent from my iPhone > > On Jun 16, 2012, at 12:01 PM, Bernadette del Rosario <badzros...@yahoo.com> > wrote: > > > Hello histonetters!!!Im presently using the Tissue Tek Cryo3 Frozen > section Machine...But i dont understand why i got problems sections falling > off slides.I used different types of slides non adhesive,adhesive(poly l > lysine,thermoshandon,starfrost sakura)..Regular procedure : Sections taken > from 2-4 microns,transfered to 95% alcohol fixative, wolfsgang fixative > etc..then after distilled water wash out...sections started to peel > off...My machine setting- chamber is -30 C;cryochamber is -39 C..example of > tissue- fibroma and salivary gland... > > I used Leica cryostat previously but i never got these problems...Even i > used non adhesive but sections still on the slides..Can someone advised > please.Maybe youre using the same tissue tek cryo 3... > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet