Michelle -
I agree that the light green is "dead" and you should make some new or buy a commercially made solution. However, if you want to try a different counterstain try a light hematoylin (usually used for glycogen) or metanil yellow. Either will give good contrast to the pink of the PAS. Cheryl Crowder _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet