In addition to Rene's comment, to cut coagulated tissue (skin that have new wound crust) and calcified tissue is difficult.
On Wed, Aug 8, 2012 at 6:45 AM, Megha Kumar <meg...@g.clemson.edu> wrote: > Hi All > I am trying to section adult mouse intestine and skin using paraffin > embedding. However, when i section, the tissue is torn although the rest of > the paraffin looks perfect. Please suggest why this is happening. Also, > sometimes the skin sections fall off the slides when I perform in situ > hybridization. Any ideas how to prevent this? > Please help! i am a beginner in histology and dont' know what to do! > regards > Megha > > > * > * > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Mehmet Fatih BOZKURT, DVM, PhD Afyon Kocatepe University Faculty of Veterinary Medicine Department of Pathology 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-173/237 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
