_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
We are beginning a retrograde tracing study in rats. We injected DiD
into the rats and in 4 weeks we will perfuse and harvest the brainstems.
My question is, is vibratome sectioning better than cryostat sectioning?
The literature that accompanied the dye said either is an option, but
some have noted dye leaching out when cyrostat sectioned. How common is
this problem? If vibratome is the better choice, is there a good
embedding protocol?
