Joe, We are abiding by the CAP 10/10 guidelines when at all possible and then we compare our results with another method or same method but other lab, i.e. reference or another clinical lab willing to trade slides with us. The comparison part is where we are having issues as we, not me personally, don't want to pay a reference lab for the comparison work so we rely on others in our "Histonet family" willing to run our slides. And of course, we're squeezing as many cases on a single slide as possible.
As to your question about 5/5 or more, CAP leaves it up to each lab as to whether its feasible and possible to obtain their recommended quota. Interesting thread as my days are spent in the middle of this exercise. Linda Sebree -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Tuesday, September 25, 2012 5:35 PM To: 'Vanessa Perez'; 'Vickroy, Jim'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Changing from Ventana IView Detection Kit toVentana Ultraview kit We are having a lively discussion about having 10 known positives and 10 known negatives to validate new antibodies. Many years ago we set up 5 and 5 even before CAP thought of the idea. This year's checklist added the 10 and 10 part, but it is up to the medical director. What is everyone else doing out there? We are using the Ventana UltraView detection kits. Everyone who uses these kits know how expensive they are. Is 5 and 5 sufficient or should go by CAP recommendations? Joe Nocito -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vanessa Perez Sent: Tuesday, September 25, 2012 2:37 PM To: Vickroy, Jim; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Changing from Ventana IView Detection Kit to Ventana Ultraview kit As far as lot to lot validation that's all we do. Use same control and compare both. Now validating a new detection kit is a whole different story. Here I just made a checklist of all the antibodies we do and had the doc sign off on each stain with the new kit. If you want you can do a slide of each with same control one with the iview and one with the ultraview. All depends on how your doc wants to validate it. Vanessa -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Tuesday, September 25, 2012 1:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Changing from Ventana IView Detection Kit to Ventana Ultraview kit We are trying to decide how to validate our stains when we switch from Ventana's IView kit to their Ultraview Kit. I have reviewed the CAP question on this and find the following wording: The performance of new lots of antibody and detection system reagents are compared with old lots before or concurrently with being placed into service. Note: Parallel staining is required to control for variables such as disparity in the lots of detection reagents or instrument function. New lots of primary and detection reagents must be compared to the previous lot using an appropriate panel of control tissues. This comparison must be made on slides cut from the same control block. Evidence: Written procedure and records of verification of new reagent lots. For new lots of antibodies we have been running the new lot and comparing with the previous lot by reviewing the control slide from the old lot to the new lot. Is this sufficient? Wording that bothers me is "appropriate panel of tissues" Thanks for your input. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet