Is there any evidence, that NBF-fixation for 72 hours alters Her2 staining, so that there's a diagnostic difference (pos./neg.)? publications with data? Personal experience? I have the feeling, that there is a mythos of overfixation. Anybody else ...? I saw turning up and down of Her2 results due to non-standardized staining-protocols. But I think a protocol, that fits to a 48 hours-fixed tissue fits also to 72 hours.
Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet