Is there any evidence, that NBF-fixation for 72 hours alters Her2 staining, so 
that there's  a diagnostic difference (pos./neg.)? publications with data?
Personal experience?
I have the feeling, that there is a mythos of overfixation. Anybody else ...?
I saw turning up and down of Her2 results due to non-standardized 
staining-protocols.  
But I think a protocol, that fits to a 48 hours-fixed tissue fits also to 72 
hours.

Gudrun Lang


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