I think you normally use ATPase at different pH's, which is an enzyme stain, to differentiate muscle fibers.
At pH 10.2 it would stain Type I fibers light and then Type II Fibers dark (all types IIa, IIb and IIc) the other pH's stratify out the type II fibers. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of arjun ruhella Sent: Monday, October 22, 2012 11:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fwd: MIXED fiber type identification by Immunohistochemistry Dear all, Its been a while i asked for a doubt to all histology experts around US, but i have not been able to get much reply (only 1 :(). I thought my doubt/e mail might have been missed by some, so sending it again. I would really appreciate if anybody could guide me in my problem. Hope to hear people's advices this time. Thanks Best, Arjun Begin forwarded message: > From: arjun ruhella <ruhella.ar...@gmail.com> > Date: October 2, 2012 8:34:59 AM EDT > To: histonet@lists.utsouthwestern.edu > Subject: MIXED fiber type identification by Immunohistochemistry > > Greetings to all, > > In immunohistochemistry, I am encountering confusion in designating muscle > fibers (based on staining color intensity) to a particular fiber type. > > This problem is particularly bothersome when i encounter the fibers stained > with 2 different colors, one color has good intensity while other seems to be > of light intensity. I don't have any criterion whether to consider this light > staining as actual staining, which would classify the fibers as MIXED fibers > or should i consider this light staining as background/non specific staining > in which case the fibers will be classified as PURE fibers. > > Does anybody can suggest any criterion/tips they have been using to identify > muscle fiber type as correctly as possible? I can understand that identifying > MIXED fibers is subjective in immunohistochemistry, but it would be of > immense help if i could get to know of any objective suggestions. > > > Animal= spinal cord injured Rat > Muscle = Soleus > Section Thickness = 10µm > > I will really appreciate the help! > > Thanks, hope to hear from ppl soon! > > BEST, > > AR Best, Arjun _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet