Deloris, We recently had what we think was a contaminated dispenser of HP from VMS. It had brown particulate matter scattered over the slide which the docs said was not bugs. Received a new dispenser from VMS and have had no problem since.
Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Deloris Carter Sent: Monday, November 05, 2012 5:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Background staining on H pylori Hi, I'm getting a lot of background staining on my HP's. We use a Ventana BenchmarkXT with ultraView DAB. The problem seems to be escalating of late, and I'm not sure why. We use Hollande's on our GI biopsies, and run them on a shorter run. Nothing has changed in the processing of the specimens, or the IHC procedure. The antibody dispenser is a newer lot, but not brand new, as we get the larger size due to the high volume of HP's we run. Any ideas? Deloris Carter HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
