We processed about 30,000 blocks in 2011 with 2 FTE Histotechs and 1 PRN tech. I also work the bench about 70% of the time, and we have a FT tech assistant.
FYI, there was a study by Kohl et al: "The CAP<NSH Workload Study" published in the Archives of Pathology, Vol 135, June 2011 that addresses this issue. Diana Goodwin Histology Supervisor RWJUHH 609-631-6996 dgood...@rwjuhh.edu -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Wednesday, November 14, 2012 11:27 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 108, Issue 18 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. On call (Martin, Gary) 2. RE: staffing numbers (Lynette Pavelich) 3. Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! (Jennifer MacDonald) 4. Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! (z o n k e d) 5. Re: staffing numbers (Rene J Buesa) 6. Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! (Rene J Buesa) 7. liftin tissue (Lee Loss) 8. Problem with cardiomyocytes staining (Amos Brooks) 9. Cassette and slide labelling systems (Sheila Adey) 10. RE: Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! (Edwards, Richard E.) 11. Technovit 9100 new fails to polymerize (Jean-Philippe Berteau) 12. Technovit 9100 new fails to polymerize (Jean-Philippe Berteau) 13. RE: staffing numbers (Tom McNemar) 14. RE: Technovit 9100 new fails to polymerize (Marsh, Nannette) 15. TBS Slide Printer (CHRISTIE GOWAN) 16. RE: liftin tissue (Vanessa Perez) 17. Zinc formalin as primary fixative for TEM (Carla M Conway) 18. H&E Question (Jaclyn Pitts) 19. RE: H&E Question (Craven Peter (NHS HIGHLAND)) 20. RE: H&E Question (Goins, Tresa) 21. Histology Supervisor Needed in Atlanta. Can you help? (Pam Barker) 22. Re: H&E Question (Rene J Buesa) ---------------------------------------------------------------------- Message: 1 Date: Tue, 13 Nov 2012 10:07:36 -0800 From: "Martin, Gary" <gmar...@marshallmedical.org> Subject: [Histonet] On call To: <histonet@lists.utsouthwestern.edu> Message-ID: <6ED9D4252F278841A0593D3D788AF24C179597D6@mailsvr.MARSHMED.local> Content-Type: text/plain; charset="us-ascii" This question may be a bit off subject Histonet, but I have not been able to find information on this subject. Our facility has decided to pay our Pathologist for their on call duties. I have been tasked with finding what other facilities are paying for this service. Any information would be appreciated. Thank You Gary ------------------------------ Message: 2 Date: Tue, 13 Nov 2012 18:54:05 +0000 From: Lynette Pavelich <lpave...@hurleymc.com> Subject: [Histonet] RE: staffing numbers To: "Hutton, Allison" <ahut...@dh.org>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <89f4666a496dc949a819ecc40e11c867bf569...@exchangemb1.hmc.hurleymc.com> Content-Type: text/plain; charset="us-ascii" We process ~14.0K cases/yr and 34.5K blocks/yr. We have 4 techs with me working part time on the bench. Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810.262.9948 mobile: 810.444.7966 ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Hutton, Allison [ahut...@dh.org] Sent: Tuesday, November 13, 2012 11:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] staffing numbers Hi Everyone, I am looking for some crude numbers regarding staffing. I would like to know the number of cases done per year and your number of histotechs. Any information will be appreciated Thank you Allison _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Tue, 13 Nov 2012 11:01:14 -0800 From: Jennifer MacDonald <jmacdon...@mtsac.edu> Subject: [Histonet] Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! To: z o n k e d <zon...@gmail.com> Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu>, "histonet-boun...@lists.utsouthwestern.edu" <histonet-boun...@lists.utsouthwestern.edu> Message-ID: <of088521f9.9dedeb08-on88257ab5.00687433-88257ab5.00687...@mtsac.edu> Content-Type: text/plain; charset="US-ASCII" if there was fat replacement, such as cirrhosis, you will see it in the morphology. From: z o n k e d <zon...@gmail.com> To: Jennifer MacDonald <jmacdon...@mtsac.edu> Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu>, "histonet-boun...@lists.utsouthwestern.edu" <histonet-boun...@lists.utsouthwestern.edu> Date: 11/13/2012 09:43 AM Subject: Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! We just wanted to see general lipids, nothing in particular. This mouse died unexpectedly and may have been part of a group that was put on a high fat or high bile acid diet and we just wanted to see what happened. On Tuesday, November 13, 2012, Jennifer MacDonald wrote: It depends on what you are using the oil red o for. Lipofuscin and ceroid can be demonstrated with an oil red o stain after processing. Jennifer MacDonald From: z o n k e d <zon...@gmail.com> To: "histonet@lists.utsouthwestern.edu" < histonet@lists.utsouthwestern.edu> Date: 11/13/2012 08:53 AM Subject: [Histonet] Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! Sent by: histonet-boun...@lists.utsouthwestern.edu Hello Histonetters, First time writer, long time reader. I'm a newbie tech in academia and I was given a simple task which I think I pretty much screwed up. I should have embedded half of a mouse liver in paraffin for microtome sectioning while the other half should have been embedded in OCT for cryosectioning (for oil red o). I made the mistake last night of placing both liver halves into the tissue processor. The liver I intended for OCT embedding is now hard as wax. Is there any way to deparaffinize processed organs and may I embed them in OCT for proper cryosectioning? I imagine that the liver would get dehydrated, I would get crappy sections, and Oil Red O won't work. Any suggestions are welcome. Thank you so much, Zoe W. -- "It costs nothing to say something kind. Even less to shut up altogether." --Nathan Fillion _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- "It costs nothing to say something kind. Even less to shut up altogether." --Nathan Fillion ------------------------------ Message: 4 Date: Tue, 13 Nov 2012 14:41:06 -0500 From: z o n k e d <zon...@gmail.com> Subject: [Histonet] Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! To: Jennifer MacDonald <jmacdon...@mtsac.edu>, ryaskov...@dir.nidcr.nih.gov Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu>, "histonet-boun...@lists.utsouthwestern.edu" <histonet-boun...@lists.utsouthwestern.edu> Message-ID: <CAE7HDS6JtL=8hw4jd0hbdytcrf9h7k_kevnesxyknvdwhwu...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Thank you all for your helpful responses! I will go ahead and carry on with paraffin sectioning and stain with H&E as planned. As for the Oil Red O, I'll try that too and see what happens. (Still amazed at how amazing Histonet is and how helpful you all are! Thank you very much for all your responses!) On Tue, Nov 13, 2012 at 2:01 PM, Jennifer MacDonald <jmacdon...@mtsac.edu>wrote: > if there was fat replacement, such as cirrhosis, you will see it in the > morphology. > > > > From: z o n k e d <zon...@gmail.com> > To: Jennifer MacDonald <jmacdon...@mtsac.edu> > Cc: "histonet@lists.utsouthwestern.edu" < > histonet@lists.utsouthwestern.edu>, " > histonet-boun...@lists.utsouthwestern.edu" < > histonet-boun...@lists.utsouthwestern.edu> > Date: 11/13/2012 09:43 AM > Subject: Re: Help! Liver mistakenly processed in paraffin (had to > be in OCT instead)! > ------------------------------ > > > > We just wanted to see general lipids, nothing in particular. This mouse > died unexpectedly and may have been part of a group that was put on a high > fat or high bile acid diet and we just wanted to see what happened. > > On Tuesday, November 13, 2012, Jennifer MacDonald wrote: > It depends on what you are using the oil red o for. Lipofuscin and ceroid > can be demonstrated with an oil red o stain after processing. > Jennifer MacDonald > > > > > From: z o n k e d <*zon...@gmail.com*> > To: "*histonet@lists.utsouthwestern.edu*" <* > histonet@lists.utsouthwestern.edu*> > Date: 11/13/2012 08:53 AM > Subject: [Histonet] Help! Liver mistakenly processed in paraffin > (had to be in OCT instead)! > Sent by: *histonet-boun...@lists.utsouthwestern.edu* > ------------------------------ > > > > Hello Histonetters, > > First time writer, long time reader. I'm a newbie tech in academia and I > was given a simple task which I think I pretty much screwed up. > > I should have embedded half of a mouse liver in paraffin for microtome > sectioning while the other half should have been embedded in OCT for > cryosectioning (for oil red o). I made the mistake last night of placing > both liver halves into the tissue processor. The liver I intended for OCT > embedding is now hard as wax. Is there any way to deparaffinize processed > organs and may I embed them in OCT for proper cryosectioning? I imagine > that the liver would get dehydrated, I would get crappy sections, and Oil > Red O won't work. > > Any suggestions are welcome. > > Thank you so much, > > Zoe W. > > > -- > "It costs nothing to say something kind. Even less to shut up altogether." > > --Nathan Fillion > _______________________________________________ > Histonet mailing list* > **Histonet@lists.utsouthwestern.edu** > **http://lists.utsouthwestern.edu/mailman/listinfo/histonet*<http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > > > -- > "It costs nothing to say something kind. Even less to shut up altogether." > > --Nathan Fillion > > > -- "It costs nothing to say something kind. Even less to shut up altogether." --Nathan Fillion ------------------------------ Message: 5 Date: Tue, 13 Nov 2012 11:42:46 -0800 (PST) From: Rene J Buesa <rjbu...@yahoo.com> Subject: Re: [Histonet] staffing numbers To: "Hutton, Allison" <ahut...@dh.org>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <1352835766.11849.yahoomail...@web163102.mail.bf1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Allison: The information you need is in at http://histosearch.com/rene.html Ren? J. ________________________________ From: "Hutton, Allison" <ahut...@dh.org> To: histonet@lists.utsouthwestern.edu Sent: Tuesday, November 13, 2012 11:00 AM Subject: [Histonet] staffing numbers Hi Everyone, I am looking for some crude numbers regarding staffing.? I would like to know the number of cases done per year and your number of histotechs.? Any information will be appreciated Thank you Allison _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Tue, 13 Nov 2012 11:47:42 -0800 (PST) From: Rene J Buesa <rjbu...@yahoo.com> Subject: Re: [Histonet] Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! To: z o n k e d <zon...@gmail.com>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <1352836062.62198.yahoomail...@web163106.mail.bf1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 You really screwed it up! When you placed both pieces of liver in the processor both were subjected to the effect of ethanol and probably xylene and both reagents extracted the liver fat and no matter what you try to do now, there will be not enough fat in the pieces as to even try the ORO stain. Try to get another piece. Anything you will try will not render good cryosections and no fat staining. Ren? J. ________________________________ From: z o n k e d <zon...@gmail.com> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Sent: Tuesday, November 13, 2012 11:52 AM Subject: [Histonet] Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! Hello Histonetters, First time writer, long time reader. I'm a newbie tech in academia and I was given a simple task which I think I pretty much screwed up. I should have embedded half of a mouse liver in paraffin for microtome sectioning while the other half should have been embedded in OCT for cryosectioning (for oil red o). I made the mistake last night of placing both liver halves into the tissue processor. The liver I intended for OCT embedding is now hard as wax. Is there any way to deparaffinize processed organs and may I embed them in OCT for proper cryosectioning? I imagine that the liver would get dehydrated, I would get crappy sections, and Oil Red O won't work. Any suggestions are welcome. Thank you so much, Zoe W. -- "It costs nothing to say something kind. Even less to shut up altogether." ? ? --Nathan Fillion _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Tue, 13 Nov 2012 21:21:01 +0000 From: Lee Loss <ll...@dermwisconsin.com> Subject: [Histonet] liftin tissue To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <2b99cb40ec5cc7409888a2961cbf96880c32a...@ex2010.derm.LOCAL> Content-Type: text/plain; charset="us-ascii" I need to lift and transfer one stained section from a slide and transfer it to another slide to destain the H&E and run an immuno on it. Does anyone have a procedure for doing that? Thank you. Lee ________________________________ The materials and information in this e-mail are confidential and may contain Protected Health Information covered under the HIPAA Privacy Rule. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution, or action taken in reliance on the contents of this information is strictly forbidden by law. If you have received this e-mail in error, please notify me by reply e-mail and then delete this message. Do not pass any of this information to anyone else. Thank you for your cooperation. ------------------------------ Message: 8 Date: Tue, 13 Nov 2012 17:18:51 -0500 From: Amos Brooks <amosbro...@gmail.com> Subject: [Histonet] Problem with cardiomyocytes staining To: histonet@lists.utsouthwestern.edu Message-ID: <cac95ki-7lmqqrbfhe4xj_wk4nc6hys7txdr6c4czrrrwjlh...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Hi Laura, I do this stain very frequently. It can be finickey. You should make very sure the pH is below 2.5. It could be that you have an old solution and over time these tend to drift toward a neutral pH. This will affect the staining. Sometimes the solutions need to be discarded and either re-made or have new purchased. Best of luck, Amos On Tue, Nov 13, 2012 at 1:00 PM, <histonet-requ...@lists.utsouthwestern.edu>wrote: > Message: 18 > Date: Tue, 13 Nov 2012 18:32:09 +0100 (CET) > From: "Laura Avogaro" <avog...@science.unitn.it> > Subject: [Histonet] Problem with cardiomyocytes staining > To: histonet@lists.utsouthwestern.edu > Message-ID: > <3064.192.168.178.78.1352827929.squir...@www.science.unitn.it> > Content-Type: text/plain;charset=iso-8859-1 > > Dear Histonetters, > I am new in histology so I ask if someone have experience with cardiac > tissue. Recently I performed a Picrosirius Red staining (pig atrium, 5 > microns thickness) but in some sections I noted cardiomyocytes colored > brown (instead of pale yellow).I wonder if it is probably due to the > thickness of the section or something alse(unfortunately I use an old > microtome whose probably fails to work in the correct way). > Thank you in advance > Best regards > > Laura > ------------------------------ Message: 9 Date: Tue, 13 Nov 2012 18:14:00 -0500 From: Sheila Adey <sa...@hotmail.ca> Subject: [Histonet] Cassette and slide labelling systems To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <bay154-w6023f497d8f33487919d48c6...@phx.gbl> Content-Type: text/plain; charset="iso-8859-1" Hi Everyone:I'm looking for opinions on cassette and slide labelling systems. Please share your likes and dislikes.ThanksSheila p.s Vendors Do Not call me over this email. That's very annoying ------------------------------ Message: 10 Date: Wed, 14 Nov 2012 09:00:11 +0000 From: "Edwards, Richard E." <r...@leicester.ac.uk> Subject: RE: [Histonet] Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! To: 'z o n k e d' <zon...@gmail.com>, Jennifer MacDonald <jmacdon...@mtsac.edu> Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu>, "histonet-boun...@lists.utsouthwestern.edu" <histonet-boun...@lists.utsouthwestern.edu> Message-ID: <7722595275a4dd4fa225b92cdbf174a101a7f49ac...@exc-mbx3.cfs.le.ac.uk> Content-Type: text/plain; charset="us-ascii" Perhaps all is not lost as you will be able to see on a H@E the spaces reluctantly vacated by the lipids. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of z o n k e d Sent: 13 November 2012 17:44 To: Jennifer MacDonald Cc: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Subject: [Histonet] Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! We just wanted to see general lipids, nothing in particular. This mouse died unexpectedly and may have been part of a group that was put on a high fat or high bile acid diet and we just wanted to see what happened. On Tuesday, November 13, 2012, Jennifer MacDonald wrote: > It depends on what you are using the oil red o for. Lipofuscin and > ceroid can be demonstrated with an oil red o stain after processing. > Jennifer MacDonald > > > > > From: z o n k e d <zon...@gmail.com <javascript:_e({}, 'cvml', > 'zon...@gmail.com');>> > To: "histonet@lists.utsouthwestern.edu <javascript:_e({}, 'cvml', > 'histonet@lists.utsouthwestern.edu');>" > <histonet@lists.utsouthwestern.edu<javascript:_e({}, 'cvml', > 'histonet@lists.utsouthwestern.edu');> > > > Date: 11/13/2012 08:53 AM > Subject: [Histonet] Help! Liver mistakenly processed in paraffin > (had to be in OCT instead)! > Sent by: histonet-boun...@lists.utsouthwestern.edu<javascript:_e({}, > 'cvml', 'histonet-boun...@lists.utsouthwestern.edu');> > ------------------------------ > > > > Hello Histonetters, > > First time writer, long time reader. I'm a newbie tech in academia and > I was given a simple task which I think I pretty much screwed up. > > I should have embedded half of a mouse liver in paraffin for microtome > sectioning while the other half should have been embedded in OCT for > cryosectioning (for oil red o). I made the mistake last night of > placing both liver halves into the tissue processor. The liver I > intended for OCT embedding is now hard as wax. Is there any way to > deparaffinize processed organs and may I embed them in OCT for proper > cryosectioning? I imagine that the liver would get dehydrated, I would > get crappy sections, and Oil Red O won't work. > > Any suggestions are welcome. > > Thank you so much, > > Zoe W. > > > -- > "It costs nothing to say something kind. Even less to shut up altogether." > > --Nathan Fillion > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu <javascript:_e({}, 'cvml', > 'Histonet@lists.utsouthwestern.edu');> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- "It costs nothing to say something kind. Even less to shut up altogether." --Nathan Fillion _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Wed, 14 Nov 2012 11:07:35 +0100 From: "Jean-Philippe Berteau" <jean-philippe.bert...@tu-harburg.de> Subject: [Histonet] Technovit 9100 new fails to polymerize To: <histonet@lists.utsouthwestern.edu> Message-ID: <001001cdc24f$deaa7390$9bff5ab0$@tu-harburg.de> Content-Type: text/plain; charset="us-ascii" Hello, I got the same problem, how did you solve it ? Best regards Jean-Philippe ------------------------------ Message: 12 Date: Wed, 14 Nov 2012 11:13:58 +0100 From: "Jean-Philippe Berteau" <jean-philippe.bert...@tu-harburg.de> Subject: [Histonet] Technovit 9100 new fails to polymerize To: <histonet@lists.utsouthwestern.edu> Message-ID: <001d01cdc250$c32ed150$498c73f0$@tu-harburg.de> Content-Type: text/plain; charset="us-ascii" Hello, As I wrote you some weeks ago, we started to use Technovit 9100 New for tissue embedding. You helped me to solve my previous problems. And I hope, you will help me to solve my present problems. Sorry, I can explain my problem as follow : The main problem is that we are having some troubles with the polymerization of Technovit 9100 New. The polymerization mixture does not want to become hard. We filled trial moulds with 3 ml of polymerization mixture. Moulds were vacuumed and sealed. We tried to perform the polymerization reaction at -30 degrees C, -20 degrees C, -4 degrees C, +4 degrees C, +20 degrees C. Technovit 9100 New fails to polymerize. After a week, the polymerization mixture becomes gelatinous but not hard. We used the manufacturer's protocol. The preparation of the polymerization mixture was precise (I accurately prepared stock solutions several times). I cannot understand where is the problem May be it is necessary to add more activator (dibenzoyl peroxide and N,N-3,5-tetramethylaniline). May be it is necessary to mix stock solutions A and B in another proportion (not 9:1). Is it necessary to prepare the solution at 4 degrees too ? What should I do? Thank you in advance, ------------------------------ Message: 13 Date: Wed, 14 Nov 2012 05:58:25 -0500 From: Tom McNemar <tmcne...@lmhealth.org> Subject: [Histonet] RE: staffing numbers To: "'Hutton, Allison'" <ahut...@dh.org>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <E9A90E28259D2F4E84308C5E8EA8F7B4013865730209@lmhs-exchange> Content-Type: text/plain; charset="us-ascii" Allison, We are right around 7k cases/year, 20k blocks, and about 33k slides. We have 3 tech including myself as cutter and embedder. The other two tech also cover grossing, special stains, IHC, and cytology prep. We have 1 lab assistant who also helps out with grossing (doesn't do frozen) and cytology. We are also looking to hire another assistant to do filing, etc. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hutton, Allison Sent: Tuesday, November 13, 2012 11:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] staffing numbers Hi Everyone, I am looking for some crude numbers regarding staffing. I would like to know the number of cases done per year and your number of histotechs. Any information will be appreciated Thank you Allison _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ------------------------------ Message: 14 Date: Wed, 14 Nov 2012 06:43:56 -0600 From: "Marsh, Nannette" <n...@stowers.org> Subject: RE: [Histonet] Technovit 9100 new fails to polymerize To: "'Jean-Philippe Berteau'" <jean-philippe.bert...@tu-harburg.de>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <2c40e43d1f7a56408c4463fd245dddf9078ed7c...@exchmb-02.stowers-institute.org> Content-Type: text/plain; charset="us-ascii" We had the same problems. We switched to Immunobed and that is wonderful. Sets up every single time. Give it a try. Best regards, Nanne -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jean-Philippe Berteau Sent: Wednesday, November 14, 2012 4:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Technovit 9100 new fails to polymerize Hello, As I wrote you some weeks ago, we started to use Technovit 9100 New for tissue embedding. You helped me to solve my previous problems. And I hope, you will help me to solve my present problems. Sorry, I can explain my problem as follow : The main problem is that we are having some troubles with the polymerization of Technovit 9100 New. The polymerization mixture does not want to become hard. We filled trial moulds with 3 ml of polymerization mixture. Moulds were vacuumed and sealed. We tried to perform the polymerization reaction at -30 degrees C, -20 degrees C, -4 degrees C, +4 degrees C, +20 degrees C. Technovit 9100 New fails to polymerize. After a week, the polymerization mixture becomes gelatinous but not hard. We used the manufacturer's protocol. The preparation of the polymerization mixture was precise (I accurately prepared stock solutions several times). I cannot understand where is the problem May be it is necessary to add more activator (dibenzoyl peroxide and N,N-3,5-tetramethylaniline). May be it is necessary to mix stock solutions A and B in another proportion (not 9:1). Is it necessary to prepare the solution at 4 degrees too ? What should I do? Thank you in advance, _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Wed, 14 Nov 2012 15:26:26 +0000 From: CHRISTIE GOWAN <christiego...@msn.com> Subject: [Histonet] TBS Slide Printer To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <snt002-w205acee3a3ce0bfe9f89d4cae...@phx.gbl> Content-Type: text/plain; charset="iso-8859-1" I am posting a question for a friend. She is looking for feedback on the TBS Shurmark slide marking instrument. All comments are appreciated. Thanks. Christie ------------------------------ Message: 16 Date: Wed, 14 Nov 2012 09:43:00 -0600 From: Vanessa Perez <vpe...@pathreflab.com> Subject: [Histonet] RE: liftin tissue To: Lee Loss <ll...@dermwisconsin.com>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <c80b5ee351aad74dadab70fabcb78d193bcdede...@exchange.pasa.local> Content-Type: text/plain; charset="us-ascii" We use Mount quick media, which comes with a procedure for section transfer.... Vanessa -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee Loss Sent: Tuesday, November 13, 2012 3:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] liftin tissue I need to lift and transfer one stained section from a slide and transfer it to another slide to destain the H&E and run an immuno on it. Does anyone have a procedure for doing that? Thank you. Lee ________________________________ The materials and information in this e-mail are confidential and may contain Protected Health Information covered under the HIPAA Privacy Rule. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution, or action taken in reliance on the contents of this information is strictly forbidden by law. If you have received this e-mail in error, please notify me by reply e-mail and then delete this message. Do not pass any of this information to anyone else. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Wed, 14 Nov 2012 07:43:56 -0800 From: Carla M Conway <cmcon...@usgs.gov> Subject: [Histonet] Zinc formalin as primary fixative for TEM To: histonet@lists.utsouthwestern.edu Message-ID: <of5b1d51f6.85cc419c-on88257ab6.0055ff46-88257ab6.00566...@usgs.gov> Content-Type: text/plain; charset="US-ASCII" Hello everyone, A colleague has tissues which are fixed in zinc formalin and wants to examine them by transmission electron microscopy. Any thoughts regarding the use of this fixative for EM? Thanks in advance, Carla Conway Histology Technician Western Fisheries Research Center, USGS 6505 N.E. 65th Street Seattle, WA 98115-5016 USA Phone: 206-526-6282 ext. 242 Fax: 206-526-6654 E-mail: cmcon...@usgs.gov ------------------------------ Message: 18 Date: Wed, 14 Nov 2012 09:50:34 -0600 From: Jaclyn Pitts <pitts.jac...@gmail.com> Subject: [Histonet] H&E Question To: histonet@lists.utsouthwestern.edu Message-ID: <canpxt2kljr3minsh55kdxbgkyb65byxvaumhb8wcpc0o-tm...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Hi All, I am having a problem with my staining, ( well, I think its pretty good but the path says its not cark enough for her) So, I am using Azer Scientific Hematoxylin Extra and I initially started out with 2.5 min in it. I upped it to 5 min and then to 10 and 12 min. The problem is the control I have works and looks great at the 2.5 min. Besides just keeping it in the heme longer what else can I do? I have cut a few extra dummy slides today so I can try different things out with them. I am tired of hearing from the path everyday that the heme is too light for her. I am a new lab for a derm clinic and I work alone so its basiclly up to me to figure this out. Please help! Thanks! Jackie ------------------------------ Message: 19 Date: Wed, 14 Nov 2012 15:52:11 +0000 From: "Craven Peter (NHS HIGHLAND)" <peter.cra...@nhs.net> Subject: RE: [Histonet] H&E Question To: Jaclyn Pitts <pitts.jac...@gmail.com>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <20121114155324.ddd98448...@nhs-pd1e-esg101.ad1.nhs.net> Content-Type: text/plain; charset="us-ascii" Jaclyn it sounds daft but check the pH of your Eosin we found this can be strongly acidic. Peter Peter L Craven FIBMS Pathology Department Raigmore Hospital ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jaclyn Pitts [pitts.jac...@gmail.com] Sent: 14 November 2012 03:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H&E Question Hi All, I am having a problem with my staining, ( well, I think its pretty good but the path says its not cark enough for her) So, I am using Azer Scientific Hematoxylin Extra and I initially started out with 2.5 min in it. I upped it to 5 min and then to 10 and 12 min. The problem is the control I have works and looks great at the 2.5 min. Besides just keeping it in the heme longer what else can I do? I have cut a few extra dummy slides today so I can try different things out with them. I am tired of hearing from the path everyday that the heme is too light for her. I am a new lab for a derm clinic and I work alone so its basiclly up to me to figure this out. Please help! Thanks! Jackie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************************************************** This message may contain confidential information. If you are not the intended recipient please inform the sender that you have received the message in error before deleting it. Please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Thank you for your co-operation. NHSmail is the secure email and directory service available for all NHS staff in England and Scotland NHSmail is approved for exchanging patient data and other sensitive information with NHSmail and GSi recipients NHSmail provides an email address for your career in the NHS and can be accessed anywhere ******************************************************************************************************************** ------------------------------ Message: 20 Date: Wed, 14 Nov 2012 16:02:57 +0000 From: "Goins, Tresa" <tgo...@mt.gov> Subject: RE: [Histonet] H&E Question To: Jaclyn Pitts <pitts.jac...@gmail.com>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <ca4df32ed505d94bb55e95487d8e984130854...@doaisd5205.state.mt.ads> Content-Type: text/plain; charset="us-ascii" Jackie - Try a different hematoxylin - we switched to Platinum Line Modified Harris Hematoxylin - we save money and the pathologists prefer the results. Tresa -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jaclyn Pitts Sent: Wednesday, November 14, 2012 8:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H&E Question Hi All, I am having a problem with my staining, ( well, I think its pretty good but the path says its not cark enough for her) So, I am using Azer Scientific Hematoxylin Extra and I initially started out with 2.5 min in it. I upped it to 5 min and then to 10 and 12 min. The problem is the control I have works and looks great at the 2.5 min. Besides just keeping it in the heme longer what else can I do? I have cut a few extra dummy slides today so I can try different things out with them. I am tired of hearing from the path everyday that the heme is too light for her. I am a new lab for a derm clinic and I work alone so its basiclly up to me to figure this out. Please help! Thanks! Jackie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 21 Date: Wed, 14 Nov 2012 11:11:34 -0500 From: "Pam Barker" <rel...@earthlink.net> Subject: [Histonet] Histology Supervisor Needed in Atlanta. Can you help? To: "Histonet" <histonet@lists.utsouthwestern.edu> Message-ID: <046c01cdc282$b7f69520$27e3bf60$@earthlink.net> Content-Type: text/plain; charset="us-ascii" Hi Histonetters! I hope everybody is having a great day. I wanted to drop you a quick line to ask for help because I am currently recruiting for one of my best clients located in Atlanta, GA. We are looking for an experienced Histology Supervisor. We are looking for someone who is ASCP certified with at least 3 years of histology and 2 years of experience supervising a histology lab. They are offering a great salary, terrific benefits, the stability of a large company and a great group of people to work with. The help I need from you is do you know anyone that might be interested in hearing about this opportunity? If so could you please forward my e-mail to them? If you are interested in this position please contact me ASAP at rel...@earthlink.net or toll free at 866-607-3542. Remember if I hire someone you refer you will receive a referral bonus! Thank you, Pam - 866-607-3542 (866-60RELIA) - Toll Free Thank You! Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: rel...@earthlink.net www.facebook.com <http://www.facebook.com/PamBarkerRELIA> /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia ------------------------------ Message: 22 Date: Wed, 14 Nov 2012 08:20:00 -0800 (PST) From: Rene J Buesa <rjbu...@yahoo.com> Subject: Re: [Histonet] H&E Question To: Jaclyn Pitts <pitts.jac...@gmail.com>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <1352910000.32673.yahoomail...@web163101.mail.bf1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I never heard of "Azer Scientific hematoxylin" but if you can leave the sections in it during 12 minutes and it is still "weak" for your pathologist, it seems that it is a "progressive" hematoxylin, of the Mayer type. Progressive hematoxylins are somewhat tricky and have to be very fresh to work well. I have always used and preferred "regressive" hematoxylins of the Harris' type. With them you are in control of the staining when you differentiate them up to the strength?proffered by your pathologist. My advise, since it seems that you cannot satisfy your pathologist's demands,is to switch to a Harris type hematoxylin and stop fiddling with the hematoxylin you are using. I always used and preferred Harris by Richard Alan. Switch and your problems will be over. Ren? J. ________________________________ From: Jaclyn Pitts <pitts.jac...@gmail.com> To: histonet@lists.utsouthwestern.edu Sent: Wednesday, November 14, 2012 10:50 AM Subject: [Histonet] H&E Question Hi All, I am having a problem with my staining, ( well, I think its pretty good but the path says its not cark enough for her) So, I am using Azer Scientific Hematoxylin Extra and I initially started out with 2.5 min in it. I upped it to 5? min and then to 10 and 12 min. The problem is the control I have works and looks great at the 2.5 min. Besides just keeping it in the heme longer what else can I do? I have cut a few extra dummy slides today so I can try different things out with them. I am tired of hearing from the path everyday that the heme is too light for her. I am a new lab for a derm clinic and I work alone so its basiclly up to me to figure this out. Please help! Thanks! Jackie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 108, Issue 18 ***************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet