Hi Albert, The first thing that comes to mind to me is aggressive "facing" or rough cutting of the block before taking your final good section. To solve this, after facing the block, (usually at 20+ microns), I go down to my desired slide micron (4) and finish facing at the lower micron for 5-10 more times. This will remove the holes caused by the high microns.
Hope this helps, Lynette ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Santiago, Albert [albert.santi...@uphs.upenn.edu] Sent: Wednesday, November 14, 2012 1:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: holes in tissue sections From: Santiago, Albert Sent: Wednesday, November 14, 2012 1:35 PM To: 'histonet@lists.utsouthwestern.edu' Subject: holes in tissue sections Hello fellow histonetters, We are a derm lab and we are having slide quality issues, I am hoping someone can provide me with some advice on how to resolve this problem: We are experiencing "holes" in tissue sections. The holes appear mainly in the epidermis but sometimes in the dermis. I've tried a few different things but still haven't resolved. If someone has experienced or seen this please help. Any and all feedback will be appreciated. I have some sample photos for your review, if anyone would like to see what I'm talking about. Please let me know and I'll email them to you. Thank you, Albert The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
