Hi Kim, try 10cc of the prediluted Eosin (you use in H & E staining) in your last 100% before your Xylene on the processor. By putting it in the last absolute it will not wash out. Your first Xylene will end up a little pink but your eyes will not strain as much trying to fine the tiny tissue fragments. (great for thread-like prostate biopsies too) We had been using it for years & our IHC seems fine. Make sure to keep your solutions for the clean cycles maintenanced on the automatic processors to avoid clogging. The only reason we stopped was the folks we purchased the new processor from told us using it would invalidate the warranty on the new processor.(I really miss it)
Cassandra Davis cda...@che-east.org 302-575-8095 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Kim Merriam [kmerriam2...@yahoo.com] Sent: Wednesday, November 28, 2012 7:54 AM To: Histonet Subject: [Histonet] eosin in the processor Hi Everyone, Years ago, my lab used to put eosin in the processor to lightly tint the smaller mouse tissues. I can't remember which station we put it in (I think it was the 2nd 100% ethanol). Also, back then my lab didn't do any IHC; will the eosin affect any IHC that might be done (I am guessing no, but I want to be sure). Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________ Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
