Sounds like you may have a cracked vortex mixer and/or silver in your reagent dispensing line. If the later is the case new tubing will fix this issue and new vortex mixer(s) will fix the issue with mixing. Do you know how to perform a vortex mix test using the service menu functions? If so, place a drop of hematoxylin on a slide and with the lid open you will be able to see if the vortex mixers are blowing the solution counter-clockwise and clockwise. Each mixer does it in a different manner to make sure the reagents are mixed well over the entire slide.
Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wben...@cua.md ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar [tmcne...@lmhealth.org] Sent: Tuesday, December 04, 2012 12:53 PM To: 'Cheryl'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Trichrome Same problem here. Using the Nexus stainer and have also experienced this with the Reticulin as well. Called service today and they elected to send an application specialist instead of a service tech. Will see what happens. Would not have expected to see this when doing the stain by hand.... Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Tuesday, December 04, 2012 8:19 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Trichrome Hi- need help troubleshooting a trichrome. The stain on the Nexus stainers is inconsistent. Taking it off the stainer and doing it by hand with a kit has similar results. The tissue is NBF fixed needle biopsies on kidney. There is a little mechanical damage on the edges of the tissue but the collagens are staining too light and the red overstains. The normal tissue as control is beautiful -- I know I know--don't force the stain--but it doesn't make sense to have this much variation from a kit or auto stainer... Help? Cheryl Kerry, HT(ASCP) Path Group of Louisiana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
