We have been using this protocol on mostly mouse tissues with great results! We run this on a VIP 3000.
70% Ethanol, room temp, 1 hour 100% Isopropyl Alcohol, room temp, 1 hour 100% Isopropyl Alcohol, room temp, 1 hour 100% Isopropyl Alcohol, room temp, 1 hour 5:1 Iso:Mineral Oil, 50 degrees, 1 hour 2:1 Iso:Mineral Oil, 50 degrees, 1 hour 100% Mineral Oil, 50 degrees, 1 hour 100% Mineral Oil, 50 degrees, 1 hour 100% Mineral Oil, 50 degrees, 6 hours Paraffin, 60 degrees, 1 hour Paraffin, 60 degrees, 1 hour Paraffin, 60 degrees, 1 hour Followed by a cleaning cycle after cassettes are removed and put into molten paraffin at the embedding center Lyn M. Stadler, BS, HTL(ASCP)CM Research Histotechnologist Department of Histopathology Cleveland Biolabs, Inc. 73 High Street Buffalo, NY 14203 716-849-6817, ext 417 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Wednesday, December 05, 2012 1:05 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 109, Issue 6 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: automated microtomes (Lynette Pavelich) 2. HistoTALK (David Kemler) 3. RE: Tissue Processors (Marcum, Pamela A) 4. RE: Tissue Processors (Boyd, Debbie M) 5. Re: Immunohistochemical detection of cytokines in older frozen sections (Rob Day) 6. RE: Histonet Digest, Vol 109, Issue 5 (Riesen, Rebecca) 7. Re: Fixation time (Geoff) 8. Histology Positions in Little Rock AR (Marcum, Pamela A) 9. Re: Fixation time (Teri Johnson) 10. Re: Tissue Processors (Teri Johnson) 11. job opening in PA (Hutton, Allison) 12. Re: Re: Tissue Processors (Rene J Buesa) 13. RE: Re: Tissue Processors (Ellenburg, Deborah) ---------------------------------------------------------------------- Message: 1 Date: Wed, 5 Dec 2012 12:10:06 +0000 From: Lynette Pavelich <lpave...@hurleymc.com> Subject: RE: [Histonet] automated microtomes To: Rene J Buesa <rjbu...@yahoo.com>, "Rathborne, Toni" <trathbo...@somerset-healthcare.com>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <89f4666a496dc949a819ecc40e11c867bf56c...@exchangemb1.hmc.hurleymc.com> Content-Type: text/plain; charset="iso-8859-1" I am purchasing my second Leica RM2255 automated microtome. Rene' is correct in saying that the carpal tunnel syndrome will eventually affect every advanced tech by using any microtome manually. On the RM2255, the flywheel additionally is automated. You can choose to use the flywheel manually or automated by the simultaneous pushing of two buttons. Nice feature for us "oldies" to ease us into automation or if you have a tiny specimen that you need to take extra care with and want to use manually. Companies are very happy to send in a demo to try for a couple weeks. Happy shopping!! ;) Lynette ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Rene J Buesa [rjbu...@yahoo.com] Sent: Tuesday, December 04, 2012 1:19 PM To: Rathborne, Toni; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] automated microtomes The advantage of the so called automated microtomes (the only thing automated about them is the block advance) is that they alleviate wrist effort and in some ways prevent carpal tunnel syndrome that affects some histotechs (mostly of the "senior persuasion"). I would go with the Leica. Ren? J. From: "Rathborne, Toni" <trathbo...@somerset-healthcare.com> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Sent: Tuesday, December 4, 2012 11:48 AM Subject: [Histonet] automated microtomes I'm looking for some opinions about the automated microtomes currently available. Which ones do most techs prefer? Which are more reliable? Is there an advantage to having a semi-automated microtome? Thanks in advance for your replies. Toni _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Wed, 5 Dec 2012 04:28:54 -0800 (PST) From: David Kemler <histot...@yahoo.com> Subject: [Histonet] HistoTALK To: Fellow HistoNetters <Histonet@Lists.UTSouthwestern.edu> Message-ID: <1354710534.83756.yahoomail...@web121504.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hello HistoNetters - ? In case you missed our last two guests on HistoTALK http://www.histotalk.com/, they were Region VII Director, ANDI GRANTHAM and KATHERINE (Kaspar)?KASPRZYK, President of the Arizona Society for Histotechnology. Just wanted to let you know. ? Yours, David ------------------------------ Message: 3 Date: Wed, 5 Dec 2012 13:00:57 +0000 From: "Marcum, Pamela A" <pamar...@uams.edu> Subject: RE: [Histonet] Tissue Processors To: "'susan.wal...@hcahealthcare.com'" <susan.wal...@hcahealthcare.com>, "rjbu...@yahoo.com" <rjbu...@yahoo.com>, "twheel...@mclean.harvard.edu" <twheel...@mclean.harvard.edu>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <41d3a1af6fef0643bdc89e0516a6ea328e5d5...@mail2node2.ad.uams.edu> Content-Type: text/plain; charset="iso-8859-1" We have 4 Thermo Excelsiors and prefer them to the VIP we no longer use. It is ease of changing reagents and programming as well as working better for our tissues. We also have Leica ASP300 that is used for only one type of tissue so we no longer need to change it weekly and expose personnel to the fumes of the reagents and possible spills. VIP are great units just not as easy to work with not that other options have become available. Pam Marcum -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of susan.wal...@hcahealthcare.com Sent: Wednesday, December 05, 2012 1:49 AM To: rjbu...@yahoo.com; twheel...@mclean.harvard.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Tissue Processors VIP always! -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, December 04, 2012 3:57 PM To: Tim Wheelock; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Tissue Processors I always used VIP because of reliability, "toughness" and customer service. Ren? J. From: Tim Wheelock <twheel...@mclean.harvard.edu> To: histonet@lists.utsouthwestern.edu Sent: Tuesday, December 4, 2012 3:48 PM Subject: [Histonet] Tissue Processors Hi Everyone: I am currently evaluating three tissue processors. They are the Sakura VIP6, the Leica ASP 6025, and the Thermo-Fisher Excelsior ES. I was wondering if people could give me their critical opinions and preferences on these three machines. In addition to reliability and ease of use,? I am interested in people's experience with tech support, software, or any other factor-positive or negative-that prompted your decision. I currently have a 14 year old Shandon Hypercenter XP. Thank you, Tim Wheelock Neuropathology Laboratory Harvard Brain Bank McLean Hospital Belmont, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 4 Date: Wed, 5 Dec 2012 13:35:29 +0000 From: "Boyd, Debbie M" <dkb...@chs.net> Subject: RE: [Histonet] Tissue Processors To: Tim Wheelock <twheel...@mclean.harvard.edu>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <7eafe982e328304da6ce2b677bb762467949f...@tn001wexmbx11.us.chs.net> Content-Type: text/plain; charset="us-ascii" Excelsior> Great workhorse. Very little to no down time. Less tech time needed as reagents dump and rotate automatically. User friendly. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tim Wheelock Sent: Tuesday, December 04, 2012 3:48 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Processors Hi Everyone: I am currently evaluating three tissue processors. They are the Sakura VIP6, the Leica ASP 6025, and the Thermo-Fisher Excelsior ES. I was wondering if people could give me their critical opinions and preferences on these three machines. In addition to reliability and ease of use, I am interested in people's experience with tech support, software, or any other factor-positive or negative-that prompted your decision. I currently have a 14 year old Shandon Hypercenter XP. Thank you, Tim Wheelock Neuropathology Laboratory Harvard Brain Bank McLean Hospital Belmont, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ------------------------------ Message: 5 Date: Wed, 5 Dec 2012 08:44:45 -0500 From: Rob Day <r...@foliobio.com> Subject: Re: [Histonet] Immunohistochemical detection of cytokines in older frozen sections To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <eef5ca71-8195-4607-96c2-75893a3a0...@foliobio.com> Content-Type: text/plain; charset=iso-8859-1 Folio biosciences is testing a process that will rejuvenate older sections to restore degraded antigenicity for ISH. It's designed to help scientists with exactly the problem you are describing Email me if you want to help us test this process using your slides. No charge of course. Rob Day. On Dec 4, 2012, at 6:26 PM, Darius Alex Schneider <schn...@gmail.com> wrote: > Dear all, > > I am trying to detect cytokines in human pancreatic sections from > diabetics. The blocks are at least 1y old, the sections are usually fresh. > I am fixing the slides with 2% PFA and permeabilizing with 0.1% saponin, > staining overnight in BSS-saponin with the primary, biotinylated antibody > and subsequently tyramid signal amplification and fluorescein based > visuatlisation. However, my results are very poor. > I am mainly interested in these cytokines: > IL1beta > IL1r > IL2 > IL6 > IL10 > IL17 > IL21 > TNFalpha > IFNgamma > > Is there somebody out there who is experienced in detecting these > cytokines, and could provide me with a comprehensive protocol? Also, who > knows what the best positive controls might be? > > Thank you so much, > > Darius > La Jolla, USA > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet Rob Day Business Development Folio Biosciences 1476 Manning Pkwy, Powell, Ohio 43065 Direct Line: (614) 407-4547 | Main Office Phone: (614) 846-2809 | Fax: (877) 591-1815 http://foliobio.com www.linkedin.com/in/robdaybiotech This message, including any attachments, is confidential and may be privileged or may contain health information protected by state and federal law. Information and opinions expressed in this message and/or attachments are those of the author and are not necessarily those of the company. If you are not the intended recipient, please notify the sender and delete this message from your system. Any use of this information by individuals other than the intended recipient is strictly prohibited. ------------------------------ Message: 6 Date: Wed, 5 Dec 2012 13:47:44 +0000 From: "Riesen, Rebecca" <rebecca.rie...@hma.com> Subject: [Histonet] RE: Histonet Digest, Vol 109, Issue 5 To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <df15acc900ddf741870c8ddcd3c9159a273ca...@hmambx02.hma.com> Content-Type: text/plain; charset="us-ascii" I am in love with the Excelsior from the standpoint of hands off daily maintenance. Waste paraffin is pumped into a container with a liner, you let it solidify and remove waste in the bag for easy disposal. Pour a couple bags of paraffin pellets in and walk away. Take a bottle of waste alcohol or xylene the instrument has pumped into off and replace it with a new bottle. Excelsior will aspirate the new fluid on the next run. You don't even have to pour into another container. The Excelsior takes the fluids right out of the manufacturer's bottle. It is so simple! If you can read, that's all you need. The screen prompts everything. I also find my solutions last longer. ------------------------------ Message: 18 Date: Tue, 4 Dec 2012 15:39:11 -0800 From: Davide Costanzo <pathloc...@gmail.com> Subject: Re: [Histonet] Tissue Processors To: Tim Wheelock <twheel...@mclean.harvard.edu> Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <1628083238193856494@unknownmsgid> Content-Type: text/plain; charset=ISO-8859-1 The excelsior is getting rave reviews from folks I know that use it. The University of Miami just ordered a few of them from my understanding. Might want to call there and ask their opinion. Personally, I think Sakura is losing ground and for good reason. We use exclusively Thermo branded equipment here. STP420 and Pathcentre. Both are great. ------------------------------ Message: 7 Date: Wed, 05 Dec 2012 09:51:53 -0500 From: Geoff <mcaul...@umdnj.edu> Subject: Re: [Histonet] Fixation time To: histonet@lists.utsouthwestern.edu Message-ID: <50bf5f89.20...@umdnj.edu> Content-Type: text/plain; CHARSET=US-ASCII; format=flowed I agree with Rene. Geoff On 12/3/2012 12:00 AM, Daniela Bodemer wrote: > Hi all, > > > > Our tissue processor has been shut down due to a contamination issue and > now all the tissues (mice and rat pelves) collected prior to this > happening have been sitting in 4% PFA. Some tissues more than a week, > when we usually fix for 48 hours. > > Now we are transferring the tissues to 70% Ethanol and they will sit > there until further notice. I am concerned about this process and what > it will do to the tissue and would like your thoughts on this. > > > > Many thanks, > > > > DB > > Research Assistant > > > > > > > ______________________________________________________________________ > This email has been scanned by the Symantec Email Security.cloud service. > For more information please visit http://www.symanteccloud.com > ______________________________________________________________________ > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcaul...@umdnj.edu ********************************************** ------------------------------ Message: 8 Date: Wed, 5 Dec 2012 16:09:20 +0000 From: "Marcum, Pamela A" <pamar...@uams.edu> Subject: [Histonet] Histology Positions in Little Rock AR To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <41d3a1af6fef0643bdc89e0516a6ea32924b5...@mail2node2.ad.uams.edu> Content-Type: text/plain; charset="us-ascii" We have two positions open for either a registered HT or HTL at UAMS. We are in Little Rock Arkansas in the middle of the state and it is a gorgeous area. We have great benefits and a competitive salary range. We are currently reorganizing the Histology Laboratory and seeing increases in our workload. This is a modern laboratory with up to date equipment and more changes coming. Join us for a new direction in your career and future. Please e-mail if you are interested and we will set up an interview either by phone for those out of town or in person after your application has been processed through our HR. (Please e-mail me first as they tend to not get all qualified applicants to us.) Please note recruiters need answer this as we are not allowed to use your services and it would be a waste of your time and energy. (Wish we could - the state says NO) Best Regards, Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 9 Date: Wed, 5 Dec 2012 16:32:43 +0000 From: Teri Johnson <tjohn...@gnf.org> Subject: [Histonet] Re: Fixation time To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <9f3cfee76e51b64991c7485270890b400ce12...@ex4.lj.gnf.org> Content-Type: text/plain; charset="us-ascii" Hi Daniela, We have successfully stored in 70% ethanol without issue. The only published account I have seen of any issue with alcohol storage is it causes vacuolization in brain. I would not really want to store in fixative any longer because you will continue creating cross links and that could introduce variation in your experimental outcome. Be very critical of the staining results you obtain on these samples compared to your others. Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 ------------------------------ Message: 10 Date: Wed, 5 Dec 2012 16:37:56 +0000 From: Teri Johnson <tjohn...@gnf.org> Subject: [Histonet] Re: Tissue Processors To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <9f3cfee76e51b64991c7485270890b400ce12...@ex4.lj.gnf.org> Content-Type: text/plain; charset="us-ascii" Dear Tim, Here's the deal, they all work. When buying new, I bought the VIP5 due to its great reputation. It was and still is a workhorse. Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 ------------------------------ Message: 11 Date: Wed, 5 Dec 2012 11:42:21 -0500 From: "Hutton, Allison" <ahut...@dh.org> Subject: [Histonet] job opening in PA To: <histonet@lists.utsouthwestern.edu> Message-ID: <38a56c4f4630d348a50b3720409270870e0fe...@dhmail.dhorg.org> Content-Type: text/plain; charset="iso-8859-1" > Doylestown Hospital is Doylestown, PA has the following position open: > > Job Title: Histology Tech - 1123 > Req: 2012-2409 > Location: > Department: Laboratory > Schedule: Mon-Fri > varied start times 0530,0630,0730 > > Job Description: > PURPOSE OF JOB: To assist the Histology and cytology Department in the > preparation of tissue and cells for microscope pathology. Performs > Histologic and Cytologic procedures as states in the Histology and Cytology > Procedure Manuals. ESSENTIAL FUNCTIONS: 45% 1. Properly orients, embeds and > sections tissues at 5 microns without artifacts or contaminants. 25% 2. > Performs routine, special, frozen and immunohistochemical staining. Manually > prepares all stock and working solutions needed to perform above stains > (Stain list is available in Histology). 20% 3. Accurately and legibly > accessions specimens with the use of the computer. Assists pathologist with > frozen sections. Follows slides through the H&E stainer and appropriately > distributes slides to the pathologists. 5% 4. Operates and maintains all > histology equipment, recycles chemicals and disposes of pathological waste, > troubleshoots outliers to help keep department running efficiently. 5% 5. > Covers the Cytology Department in the absence of the cytotechnologist. Assists Cytology and Office with designated duties. > For more information, please visit www.dh.org > > > ------------------------------ Message: 12 Date: Wed, 5 Dec 2012 08:54:40 -0800 (PST) From: Rene J Buesa <rjbu...@yahoo.com> Subject: Re: [Histonet] Re: Tissue Processors To: Teri Johnson <tjohn...@gnf.org>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <1354726480.27777.yahoomail...@web163106.mail.bf1.yahoo.com> Content-Type: text/plain; charset=utf-8 If I may, I would like to add something: do you remember the sales success of the VW Beetle? Why was it a success? Because of its simplicity. They did not even have a gasoline gage and if you ran out of gasoline there was 1 extra gallon reserve that you opened with a handle underneath the passenger's seat. No thrills, no complexity. You could repair it yourself. Try to do now with a new VW "Beetle", the new ones with the motor at the front of the car. You will not be able to do it. For the same reason there are labs that today still use the "carousel" type tissue processors, the "Technicon" type still manufactured (now by Leica and some Indian and Chinese lab manufacturers). Something similar can be said to the "basic" VIP with less valves, less automation but extremely reliable. Those more "automated" with "hands-free reagents transfer" by laws of probability have to be more prone to failure. More components = greater parts to "go wrong". Regarding lower exposure to reagents, that depends on the reagents, not on the instrument. It is well documented at this moment that the only reagents you need to process tissue and obtain the best quality possible is with innocuous reagents??in a protocol using??isopropyl alcohol ??? mineral oil ??? paraffin??neither are noxious to the techs' health. This is my humble opinion. Ren?? J. From: Teri Johnson <tjohn...@gnf.org> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Sent: Wednesday, December 5, 2012 11:37 AM Subject: [Histonet] Re: Tissue Processors Dear Tim, Here's the deal, they all work. When buying new, I bought the VIP5 due to its great reputation. It was and still is a workhorse. Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Wed, 5 Dec 2012 12:13:51 -0500 From: "Ellenburg, Deborah" <deborah_ellenb...@bshsi.org> Subject: RE: [Histonet] Re: Tissue Processors To: "Rene J Buesa" <rjbu...@yahoo.com>, "Teri Johnson" <tjohn...@gnf.org>, <histonet@lists.utsouthwestern.edu> Message-ID: <bb153a828ab3fa439fbd55c818a0ec8801a46...@edc-mail-02.ads.bshsi.com> Content-Type: text/plain; charset="UTF-8" Well stated - agree 100%. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, December 05, 2012 11:55 AM To: Teri Johnson; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: Tissue Processors If I may, I would like to add something: do you remember the sales success of the VW Beetle? Why was it a success? Because of its simplicity. They did not even have a gasoline gage and if you ran out of gasoline there was 1 extra gallon reserve that you opened with a handle underneath the passenger's seat. No thrills, no complexity. You could repair it yourself. Try to do now with a new VW "Beetle", the new ones with the motor at the front of the car. You will not be able to do it. For the same reason there are labs that today still use the "carousel" type tissue processors, the "Technicon" type still manufactured (now by Leica and some Indian and Chinese lab manufacturers). Something similar can be said to the "basic" VIP with less valves, less automation but extremely reliable. Those more "automated" with "hands-free reagents transfer" by laws of probability have to be more prone to failure. More components = greater parts to "go wrong". Regarding lower exposure to reagents, that depends on the reagents, not on the instrument. It is well documented at this moment that the only reagents you need to process tissue and obtain the best quality possible is with innocuous reagents??in a protocol using??isopropyl alcohol ??? mineral oil ??? paraffin??neither are noxious to the techs' health. This is my humble opinion. Ren?? J. From: Teri Johnson <tjohn...@gnf.org> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Sent: Wednesday, December 5, 2012 11:37 AM Subject: [Histonet] Re: Tissue Processors Dear Tim, Here's the deal, they all work. When buying new, I bought the VIP5 due to its great reputation. It was and still is a workhorse. Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone, and permanently delete the original e-mail, attachment(s), and any copies. ________________________________________________________________________________________________________ ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 109, Issue 6 **************************************** This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
