Hi Christina, First I would have you use a room temperature block. If the tissue needs to be rehydrated briefly, use room temp water instead of ice. I can get 12 micron ribbons of bone using Paraplast extra, I haven't tried thicker.
If that doesn't work, what blade are you using? In my experience, the Accuedge low profile blades tend to curl more quickly than other blades. You can try using a thicker blade or one from a different manufacturer and see if that helps. Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
