Hi Histonetters ! Can anyone suggest how to prevent adult mouse skin sections from falling off during in situ hybridization? How to make sections adhere better to slides? I am using poly-lysine coated slides for 5-10 micron thick skin sections. The tissue is fixed in 4% PFA and embedded in paraffin. Please help ! thanks megha
-- Megha Kumar, Ph.D. Post-doctoral Research Scholar Sen-Bandyopadhyay Lab Department of Biological Sciences and Bioengineering Indian Institute of Technology Kanpur Kanpur, 208016 India phone - 9695830033 * * * * _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet