Hello, I used to section the brains of my mice by microtome I did not have any issue. This period I changed to the vibratome I notice that it is cutting whole sections at the beginning then it is cutting just parts of the brain such as the hippocampus and parietal cortex without the frontal cortex or even just the hippocampus without the other structures. I am using cold PBS and sharp blade and the angle of the blade is 5 degree. If any body had any experience before with the vibratome or encounter this problem please do not hesitate to help me. Thank you so much. Regards. Narjes Baazaoui -- Narjes Baazaoui, PhD student Institute of Basic research (IBR), Laboratory of chemical neuropathology 3475995601 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet