How do I unsubscribe from Histonet?? Thank you, Arjun On Wed, Mar 27, 2013 at 1:46 PM, Kienitz, Kari <kkien...@orclinic.com>wrote:
> If your acetic acid solution is made in-house....I would suggest remaking > it, just in case it was accidently made stronger. > > > Kari Kienitz HT, (ASCP) > Histology Laboratory > Portland Gastroenterology > The Oregon Clinic > 1111 NE 99th Ave > Portland, OR 97220 > 503.935.8311 > kkien...@orclinic.com > > > > > CONFIDENTIALITY WARNING: This e-mail and any attachments are for the > exclusive and confidential use of the intended recipient. If you are not > the intended recipient, please do not read, distribute or take action in > reliance upon this missive. If you have received this in error, please > notify the sender immediately by reply e-mail and delete this message and > its attachments from your computer system. Thank you > ________________________________________ > From: histonet-boun...@lists.utsouthwestern.edu [ > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sophia Lin [ > lins0...@gmail.com] > Sent: Wednesday, March 27, 2013 9:26 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] linear stainer hematoxylin staining issues > > We have the Hacker linear stainer, and for the past two weeks we had clean, > crisp H&E staining. However, last Friday, something happened and our H&E > stains no longer look crisp, but instead have a hazy, unreadable look. > Nothing different has been done, and we have troubleshot for every possible > scenario. After a clean set up, we noticed that the hematoxylin is not > staining very well. We have it set for 2 minutes with agitation for a > uniform staining. Hematoxylin is Harris Special (mercury free) from MCC and > the eosin is with phloxine b from AMT. After the hematoxylin (2 buckets for > total of 4.5 minutes), we use 1.5% acetic acid. It seems that the > hematoxylin is not staining well. > > Any tips? We may need to consider increasing a bucket for hematoxylin? > > Thanks, > > Sophia > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
