Grossing Tech
Vanessa -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Barr,Kaye H Sent: Tuesday, April 30, 2013 12:49 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Histonet Digest, Vol 113, Issue 30 What title do most use for someone who does gross and is not a PA? -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Tuesday, April 30, 2013 12:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 113, Issue 30 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. BIG PROBLEM (Behnaz Sohrab) 2. Re: BIG PROBLEM (Linda) 3. Yearly Training (Nancy Schmitt) 4. HIER pressure cookers.... (k. leigh adams) 5. RE: HIER pressure cookers.... (Connolly, Brett M) 6. RE: HIER pressure cookers.... (Blazek, Linda) 7. Re: HIER pressure cookers.... (Jan Shivers) 8. RE: 5 gal specimen container search (Hannen, Valerie) 9. RE: 5 gal specimen container search (Shirley A. Powell) 10. RE: HIER pressure cookers.... (dingers...@aplaboratories.com) 11. AW: [Histonet] BIG PROBLEM (Gudrun Lang) 12. Processor question - alcoholic formalin vs. buffered formalin (Lake, Kim S) 13. RE: Processor question - alcoholic formalin vs. buffered formalin (Hannen, Valerie) 14. cassette labelers (Margiotta-Watz, Michele) ---------------------------------------------------------------------- Message: 1 Date: Mon, 29 Apr 2013 10:36:01 -0700 From: "Behnaz Sohrab" <sohra...@ah.org> Subject: [Histonet] BIG PROBLEM To: <histonet@lists.utsouthwestern.edu> Message-ID: <517e4d11.4347.005...@ah.org> Content-Type: text/plain; charset="us-ascii" Good Morning, We have two Leica H&E stainer( old xt-5010 and new 5020). We do use : Gill's 3 for 6 minutes, 4% Glacial ACETIC Acid 40 seconds Bluing : 45 sec. We done this for years!! last few months our Pantologists are complaining about GI Biopsies that they have TOO much blue in cytoplasma and the Mucin is too purple and blue? Please any suggestion?? Mean while we are trying the NEW kit from Leica for H&E , they think that is TOO pale?any of you has tried this new stain from Lecia? With much application. Behnaz Sohrab, Los Angeles ------------------------------ Message: 2 Date: Mon, 29 Apr 2013 11:30:38 -0700 (PDT) From: Linda <lmd...@yahoo.com> Subject: Re: [Histonet] BIG PROBLEM To: Behnaz Sohrab <sohra...@ah.org>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <1367260238.82176.yahoomail...@web164002.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi, ? I would first check with your alcohol and xylene suppliers to see if they changed anything.? When something?goes south it is usually because the manufacturer have changed.? This happened with my silver stains. ? You may have to go back to the basics- harris hematoxylin, acid alcohol, and eosin y.? Richard Allen 7211 hematoxylin and the rest of their staining is excellent in these situations until you can get it back under control.? If you send them slides they will work a time out for you. ? If you are using Leica's selectech, which is what I started this lab out with because I was heavily sampled out,?it has blue nuclei instead of the traditional purple.? I do 5 minutes in hematoxylin, 30 dips in clarifier and 1 minute in blue, 55 sec in eosin but I have (2) 95s after.? They also have a tech dept to help.? I have a Leica autostainer too. ? I hope this helps. ? Linda Dee, HT(ASCP) ? ________________________________ From: Behnaz Sohrab <sohra...@ah.org> To: histonet@lists.utsouthwestern.edu Sent: Monday, April 29, 2013 12:36 PM Subject: [Histonet] BIG PROBLEM Good Morning, We have two Leica H&E stainer( old xt-5010 and new 5020). We do use : Gill's 3 for 6 minutes, 4% Glacial ACETIC Acid 40 seconds Bluing : 45 sec. We done this for years!! last few months our Pantologists are complaining about GI Biopsies that they have TOO much blue in cytoplasma and the Mucin is too purple and blue? Please any suggestion?? Mean while we are trying the NEW kit from Leica for H&E , they think that is TOO pale?any of you has tried this new stain from Lecia? With much application. Behnaz Sohrab, Los Angeles _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Mon, 29 Apr 2013 18:39:17 +0000 From: Nancy Schmitt <nancy_schm...@pa-ucl.com> Subject: [Histonet] Yearly Training To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <906b4da90ed1db4db6c7e94d7cee6c36813c0...@peitha.wad.pa-ucl.com> Content-Type: text/plain; charset="us-ascii" Cindy- We also have a yearly competency packet. It follows JCAHO standards and includes direct observations, log documentation for QC and maintenance and problem solving through written exam. Systems covered are H&E, IHC, Special stains, processing/embedding, Cytology prep, and Misc. Histology. Nancy Schmitt Histology Coordinator Dubuque, IA ----------------------------------------------------- From: Cindy Pyse [mailto:cp...@x-celllab.com] Sent: Friday, April 26, 2013 4:49 PM To: 'histonet-boun...@lists.utsouthwestern.edu' Subject: Yearly training Happy Friday Everyone I just wanted an opinion on what everyone's facilities train for annually. An example is HIPPA training. I know what my regs require, I just wanted to see what everyone else in histoland was doing. Thanks in advance for all responses. I hope everyone has a great weekend. Finally we are going to have a good weather weekend in Western NY. Cindy NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ------------------------------ Message: 4 Date: Tue, 30 Apr 2013 09:29:33 -0400 From: "k. leigh adams" <k.leigh.adams....@gmail.com> Subject: [Histonet] HIER pressure cookers.... To: histonet@lists.utsouthwestern.edu Message-ID: <cahot8dv4sr0adagb9+ggbd6th3cbrrbs0tdrb1bu0hobrhb...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Any input as to preferred instruments would be greatly appreciated... Leigh ------------------------------ Message: 5 Date: Tue, 30 Apr 2013 09:53:50 -0400 From: "Connolly, Brett M" <brett_conno...@merck.com> Subject: RE: [Histonet] HIER pressure cookers.... To: "k. leigh adams" <k.leigh.adams....@gmail.com>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <c01c35b84dcdce49bc60867e87f1c8fe68d4403...@usctmxp51015.merck.com> Content-Type: text/plain; charset="us-ascii" Biocare Decloaker here !! Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_conno...@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of k. leigh adams Sent: Tuesday, April 30, 2013 9:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HIER pressure cookers.... Any input as to preferred instruments would be greatly appreciated... Leigh _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------ Message: 6 Date: Tue, 30 Apr 2013 09:58:58 -0400 From: "Blazek, Linda" <lbla...@digestivespecialists.com> Subject: RE: [Histonet] HIER pressure cookers.... To: "Connolly, Brett M" <brett_conno...@merck.com>, "k. leigh adams" <k.leigh.adams....@gmail.com>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <5a2bd13465e061429d6455c8d6b40e391652b87...@ibmb7exchange.digestivespecialists.com> Content-Type: text/plain; charset="us-ascii" I second that! Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lbla...@digestivespecialists.com -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M Sent: Tuesday, April 30, 2013 9:54 AM To: k. leigh adams; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HIER pressure cookers.... Biocare Decloaker here !! Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_conno...@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of k. leigh adams Sent: Tuesday, April 30, 2013 9:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HIER pressure cookers.... Any input as to preferred instruments would be greatly appreciated... Leigh _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Tue, 30 Apr 2013 09:34:56 -0500 From: Jan Shivers <shive...@umn.edu> Subject: Re: [Histonet] HIER pressure cookers.... To: "Blazek, Linda" <lbla...@digestivespecialists.com> Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu>, "k. leigh adams" <k.leigh.adams....@gmail.com> Message-ID: <caeoc1q3tgnqtbbqyulkzwh2symq+mbylnf_0pgd18suo+4+...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Third that. Jan S On Tue, Apr 30, 2013 at 8:58 AM, Blazek, Linda < lbla...@digestivespecialists.com> wrote: > I second that! > > Linda Blazek HT (ASCP) > Manager/Supervisor > GI Pathology of Dayton > Digestive Specialists, Inc > Phone: (937) 396-2623 > Email: lbla...@digestivespecialists.com > > > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu [mailto: > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M > Sent: Tuesday, April 30, 2013 9:54 AM > To: k. leigh adams; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] HIER pressure cookers.... > > Biocare Decloaker here !! > > Brett M. Connolly, Ph.D. > Principal Scientist, Imaging Dept. > Merck & Co., Inc. > PO Box 4, WP-44K > West Point, PA 19486 > brett_conno...@merck.com > T- 215-652-2501 > F- 215-993-6803 > > > > > > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu [mailto: > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of k. leigh adams > Sent: Tuesday, April 30, 2013 9:30 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] HIER pressure cookers.... > > Any input as to preferred instruments would be greatly appreciated... > > Leigh > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Notice: This e-mail message, together with any attachments, contains > information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New > Jersey, USA 08889), and/or its affiliates Direct contact information for > affiliates is available at > http://www.merck.com/contact/contacts.html) that may be confidential, > proprietary copyrighted and/or legally privileged. It is intended solely > for the use of the individual or entity named on this message. If you are > not the intended recipient, and have received this message in error, please > notify us immediately by reply e-mail and then delete it from your system. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Jan Shivers Senior Scientist IHC/Histology Section Head Pathology Teaching Program UMN Veterinary Diagnostic Laboratory University of Minnesota College of Veterinary Medicine 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu ------------------------------ Message: 8 Date: Tue, 30 Apr 2013 10:36:24 -0400 From: "Hannen, Valerie" <valerie.han...@parrishmed.com> Subject: RE: [Histonet] 5 gal specimen container search To: 'Jackie O'Connor' <b427...@aol.com>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <450B7A81EDA0C54E97C53D60F00776C3231B3D7C30@isexstore03> Content-Type: text/plain; charset="us-ascii" Jackie... We have ordered these from a company by the name of Centurian Medical. The lids are sold separately. Valerie -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jackie O'Connor Sent: Wednesday, April 24, 2013 3:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] 5 gal specimen container search Anyone in histoland know a vendor where I can find a 5 gallon leakproof bucket, drum, pail for shipping a large pathology specimen? Jackie O' _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet =================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" =================== ------------------------------ Message: 9 Date: Tue, 30 Apr 2013 10:38:59 -0400 From: "Shirley A. Powell" <powell...@mercer.edu> Subject: RE: [Histonet] 5 gal specimen container search To: "Hannen, Valerie" <valerie.han...@parrishmed.com>, 'Jackie O'Connor' <b427...@aol.com>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <9BF995BC0E47744E9673A41486E24EE2579B14884C@MERCERMAIL.MercerU.local> Content-Type: text/plain; charset="us-ascii" You can also check with restaurant supply houses, we have purchased great large containers with lids for this purpose. Much cheaper than the lab supply houses. Shirley -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie Sent: Tuesday, April 30, 2013 10:36 AM To: 'Jackie O'Connor'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] 5 gal specimen container search Jackie... We have ordered these from a company by the name of Centurian Medical. The lids are sold separately. Valerie -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jackie O'Connor Sent: Wednesday, April 24, 2013 3:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] 5 gal specimen container search Anyone in histoland know a vendor where I can find a 5 gallon leakproof bucket, drum, pail for shipping a large pathology specimen? Jackie O' _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ============= "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ============= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Tue, 30 Apr 2013 07:40:51 -0700 From: <dingers...@aplaboratories.com> Subject: RE: [Histonet] HIER pressure cookers.... To: "Blazek, Linda" <lbla...@digestivespecialists.com>, "Connolly, Brett M" <brett_conno...@merck.com>, "k. leigh adams" <k.leigh.adams....@gmail.com>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <20130430074051.073ecbdb5144cf8a05e574ee22bfb11a.114d0c0283....@email17.secureserver.net> Content-Type: text/plain; charset="utf-8" dingers...@aplaboratories.com -------- Original Message -------- Subject: RE: [Histonet] HIER From: "Blazek, Linda" <[1]lbla...@digestivespecialists.com> To: "Connolly, Brett M" <brett_conno...@merck.com>, " adams" <[2]k. "[3]histonet@lists.utsouthwestern.edu" <[4]histonet@lists.utsouthwestern.edu< I second that! Linda Blazek HT (ASCP) GI Pathology of Dayton Digestive Speciali Phone: (937) 396-2623 Email: [5]lbla...@digestivespecialists.com -----Original Message----- From: [6]histonet-bounces@lists.utsouthwes mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Connolly Sent: Tuesday, April 30, 2013 9:54 AM To: k. leigh ad Subject: RE: [Histonet] HIER pressure cookers... Biocare Decloaker here !! Brett M. Connolly, Ph Principal Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 [9]brett_conno...@merck.com T- 215-652-2501 F- 215-993-6803 -----O From: [10]histonet-boun...@lists.utsouthwestern.edu [[11]mailto:histonet-bounc k. leigh adams Sent: Tu To: [12]histonet@lists.utsouthwestern.edu Subject: [His Any input as to preferred ins appreciated... Leigh _________ Histonet mailing list [13]Histonet@lists.utsouthwe [14]http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contai ns information of Merck & Co., Inc. (One Merck Drive, Whitehouse Statio contact information [15]http://www.merck.com/contact/contacts.html) that may confidential, proprietary copyrighted and/or legally privileged. It is i this messag received this message in e-mail and then delete it fro ________________________________________ Histonet mailing list [16]Histonet@lists.utsouthwestern.edu [17]http://lists.uts ________________ Histonet mailing list [18]Histonet@lists.utsouthwestern [19]http://lists.utsouthwestern.edu/mailman/listinfo/histonet References 1. 3D"mailto:lbla 2. 3D"mailto:k.leigh.adams....@gmail.com"; 3. 3D"mailto:histonet@lists.utsout 4. file://localhost/tmp/3D"m 5. 3D"mailto:lblazek 6. 3D"mailto:h 7. 3D"mailto:histonet-boun...@lists.utsouthwestern.edu"; 8. 3D"mailto:histonet@lists.utsouthwestern.edu"; 9. 3D"mailto:bre 10. 3D"mailto:histonet-bounces@lists.u 11. ="mailto:histonet-boun...@lists.utsouthwestern.edu"; 12. 3D"mailto:histo...@lists.ut 13. 3D"mailto:Histonet@lists.utsouthwestern.edu"; 14. 3D"http://lists.utsouthwestern.edu/mailman/list 15. 3D"http://www.merck.com/conta 16. 3D"mailto:Histonet@lists. 17. file://localhost/tmp/3D" 18. ="mailto:Histonet@lists.utsouthwestern.edu"; 19. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/ ------------------------------ Message: 11 Date: Tue, 30 Apr 2013 17:15:01 +0200 From: "Gudrun Lang" <gu.l...@gmx.at> Subject: AW: [Histonet] BIG PROBLEM To: "'Behnaz Sohrab'" <sohra...@ah.org> Cc: histonet@lists.utsouthwestern.edu Message-ID: <000301ce45b5$7d292a50$777b7ef0$@gmx.at> Content-Type: text/plain; charset="iso-8859-1" Too much blue is mostly a problem of too less red. Have you checked your Eosin? pH? Rinsing after eosin? My personal issue was sometimes too thin sections. Those looked also rather pale. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Behnaz Sohrab Gesendet: Montag, 29. April 2013 19:36 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] BIG PROBLEM Good Morning, We have two Leica H&E stainer( old xt-5010 and new 5020). We do use : Gill's 3 for 6 minutes, 4% Glacial ACETIC Acid 40 seconds Bluing : 45 sec. We done this for years!! last few months our Pantologists are complaining about GI Biopsies that they have TOO much blue in cytoplasma and the Mucin is too purple and blue? Please any suggestion?? Mean while we are trying the NEW kit from Leica for H&E , they think that is TOO pale?any of you has tried this new stain from Lecia? With much application. Behnaz Sohrab, Los Angeles ------------------------------ Message: 12 Date: Tue, 30 Apr 2013 15:30:33 +0000 From: "Lake, Kim S" <kim-l...@uiowa.edu> Subject: [Histonet] Processor question - alcoholic formalin vs. buffered formalin To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <f3181e1428ad274eaef67664c5b662ab25827...@itsnt443.iowa.uiowa.edu> Content-Type: text/plain; charset="us-ascii" I attended the Tri-State Symposium in Dubuque, IA last week (which was fantastic) and sat in on a "Boot Camp for Histology" workshop. At this workshop I realized that some problems we have been having with sectioning could be due to our processor set up. Before microtomy we have to soak our blocks for 10-15 minutes, and I suspect that this is because we are over dehydrating our tissues in the during processing. Our first solution, which the cassettes sit in for about 8 hours until the overnight processing begins, is alcoholic formalin, which is 450ml buffered formalin, 900ml water, and 3300ml 95% alcohol. Our processor is a Leica TP 1050 that has been chugging along for about 20 years now. The reason we use alcoholic formalin instead of just normal buffered formalin has been lost to the mists of time, and I was wondering what you all use as a holding solution on your processor, and why. Thanks! Kim Lake Laboratory Manager Oral Pathology Laboratory University of Iowa College of Dentistry Phone (319) 384 4433 Fax (319) 353 5569 ------------------------------ Message: 13 Date: Tue, 30 Apr 2013 11:40:53 -0400 From: "Hannen, Valerie" <valerie.han...@parrishmed.com> Subject: RE: [Histonet] Processor question - alcoholic formalin vs. buffered formalin To: "'Lake, Kim S'" <kim-l...@uiowa.edu>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <450B7A81EDA0C54E97C53D60F00776C3231B3D7C33@isexstore03> Content-Type: text/plain; charset="us-ascii" We use 10 % Neutral Buffered Formalin. It is a wonderful fixative and gives us really great fixation. It does sound like you are overly dehydrating your tissues. Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.han...@parrishmed.com -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lake, Kim S Sent: Tuesday, April 30, 2013 11:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processor question - alcoholic formalin vs. buffered formalin I attended the Tri-State Symposium in Dubuque, IA last week (which was fantastic) and sat in on a "Boot Camp for Histology" workshop. At this workshop I realized that some problems we have been having with sectioning could be due to our processor set up. Before microtomy we have to soak our blocks for 10-15 minutes, and I suspect that this is because we are over dehydrating our tissues in the during processing. Our first solution, which the cassettes sit in for about 8 hours until the overnight processing begins, is alcoholic formalin, which is 450ml buffered formalin, 900ml water, and 3300ml 95% alcohol. Our processor is a Leica TP 1050 that has been chugging along for about 20 years now. The reason we use alcoholic formalin instead of just normal buffered formalin has been lost to the mists of time, and I was wondering what you all use as a holding solution on your processor, and why. Thanks! Kim Lake Laboratory Manager Oral Pathology Laboratory University of Iowa College of Dentistry Phone (319) 384 4433 Fax (319) 353 5569 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet =================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" =================== ------------------------------ Message: 14 Date: Tue, 30 Apr 2013 16:31:49 +0000 From: "Margiotta-Watz, Michele" <mmargio...@bmhmc.org> Subject: [Histonet] cassette labelers To: "'histonet@lists.utsouthwestern.edu'" <histonet@lists.utsouthwestern.edu> Message-ID: <230d0b9ec57d7a45a7a186c6ab4c7abc296f3...@bmh-exchange-02.bmhmc.org> Content-Type: text/plain; charset="us-ascii" Hi All, We are looking to purchase 2 cassette labelers and would like some feedback on which ones you might recommend. Also, is there a difference between Thermo's Printmate and Microwriter? Looks like the Printmate is just a newer version. Your input would be greatly appreciated. Thanks, Michele Histology Supervisor BMHMC 631-654-7192 DISCLAIMER: This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 113, Issue 30 ***************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
