Hi All, I had wonderful help from this site for my Woodstain Scarlet-Acid Fuchsin binding collagen problem. Now that this is fixed I have realized that the Alcian Blue is not staining my excessively fixed samples (Human Aorta & Aortic Valve, formalin fixed 2-4 years, embedded in paraffin and stored at RT for ~6 years). I have some samples fixed for only 2-6 months and these are fine but the samples fixed for 2-4 years do not uptake the Alcian Blue. I have taken it up to 1 hr in the 1% Alcian Blue solution followed by 10 min in 56*C Alkaline Alcohol solution. I do a 1hr Bouin's 50*C to start (I have also tried Bouin's at 60*C).
Any ideas on how I can get the Alcian Blue to stain these excessively fixed samples or possibly a substitute? I need to see the proteoglycans/GAGs. Thank you Krista _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet