Hi All, Has anyone tried to section cells cut from a trans well dish? We have cells that are cultured and attach to a trans well membrane and our pathologist wants to look at them on the membrane but the membrane is a thin plastic film...see the problem. We fixed the cells in the trans well for 5 minutes then cut the trans well out of the well and bisected it and processed overnight we use paraffin type #1 paraffin. The next morning we embedded it with type #9 paraffin. The samples cut but do not stay on the slide after xylene. I think the plastic does not adhere to the slide. Would a adhesive slide work to keep the thin plastic stuck to the slide??
My pathologist does not want to change to a collagen membrane so he wants me to find another (harder paraffin) to use, I don't think that will help. Has anyone does similar processing/embedding? I'll take any help I can get.. Thanks, Jamie Abbvie Bioreseach Center Pharmacology 100 Research Dr. Worcester,Ma 01605 OFFICE +1 508-688-3134 FAX +1 508-793-4895 EMAIL jamie.erick...@abbvie.com<mailto:jamie.erick...@abbvie.com> _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet