Richard
We use 0.04% T. Blue in an acetate buffer that is pH'd to 4.0. Stain for about
5 minutes, rinse in water, let air dry and then coverslip in xylene. You will
loose the metachromatic staining if you run the slides through alcohol. I have
an SOP if you would like to see one.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
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________________________________________
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Edwards, Richard E.
[r...@leicester.ac.uk]
Sent: Friday, July 12, 2013 9:17 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] tol blue and mast cells
Hi All, your thoughts on the best toluidine blue method for mast cells?,
many thanks.
Richard Edwards
University of Leicester
U.K.
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