Dear Histonetters, I was wondering if anyone has experience with Jones' Methenamine Silver stain? We have attempted this stain several times, each time with a slight tweak to the protocols we found online. We found that it took much longer than expected (3-4 hours compared to the expected 1 hour) for the membranes to develop the expected black color every attempt we made. The few times we did see the black color develop in the membranes, the next step in the gold chloride solution (1 minute) washed away all the dark color in the kidney, leaving it a very light brown. However, we did notice that only the skin sample on our test slides retained the black color. Since we ultimately need this stain for kidney samples, this didn't do us any good. We tried thinner sections (2 micron), acid washed glassware, brand new reagents, and multiple protocols with no luck. I would greatly appreciate any advice as to what we could try differently or if anyone else has experienced these same problems.
Thank you! Anna _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet