Hi all! We had a discussion on the cause of IHC positivity/false-positivity on the edges of core needle biopsies. (eg. breast, ER)
One said, it is due to overfixation with formaldehyde-concentrations above 5%. (=false pos.) My opinion is, that's it's rather an effect of underfixation in the center in those cases. (= real pos.) In my lab, we rarely see this margin-effect and the biopsies are fixed for 6 hours at least. I searched in Pubmed for publications on this assay, but without success. Can anyone help me out with any literature? Or personal experience? Thanks in advance Gudrun Lang Ltd. BMA Histolab Linz, Austria _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
