Dear Natalie,
I can't help you with PAX8, and for Ki67 we use a Ventana pre-dilute. I can
recommend Biocare Medical polyclonal p40, cat#ACI3030B.
I use it diluted 1/100.
Regards
Gary Gracie
Senior Technical Officer
IHC Laboratory
Anatomical Pathology
St Vincents Hospital
Sydney, Australia
Hello Everyone,
I am currently looking to bring a few new antibodies in
house; KI-67,P40, and PAX8. I have not worked with these antibodies yet and
wanted to get some opinions from everyone. For P40 and PAX8; are you using a
monoclonal or polyclonal? Also what company are you getting them from? My
hospital deals mainly with Dako, Biocare, and Cellmarque. We are a very small
facility that does HIER with a waterbath, and then manual IHC pipetting with
the old sequenza coverplate tiles. So usually we prefer concentrate for
antibodies not pre-dilute because of the longer expiration times, and of course
the cost. So I guess what I am asking is, what clone do you prefer. What
company do you get it from? How much does it cost? And have you ever run it on
a manual setup, if so how was it to work with?
Thanks for all the help in advance,
Natalie J. Nagy
Histology Supervisor
Holyoke Medical Center
nagy_nata...@holyokehealth.com
**************************
**********************************************************************
This email and any files transmitted with it are confidential and
intended solely for the use of the individual or entity to whom they
are addressed. If you have received this email in error please notify
the system manager.
This footnote also confirms that this email message has been virus
scanned and although no viruses were detected by the system, St Vincents &
Mater Health Sydney accepts no liability for any consequential damage
resulting from email containing any computer viruses.
**********************************************************************
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
