I have obtained excellent immunoreactivity for some proteins on various tissues that have been in formalin for more than a year; however, you won't know unless you try it and there are "internal positive controls" to validate negative results.
Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax richard.car...@hhchealth.org ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Tim Wheelock [twheel...@mclean.harvard.edu] Sent: Wednesday, October 09, 2013 4:34 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Immunostaining after prolonged formalin immersion? Hi Everyone: If tissue has been sitting in 10 % neutral buffered formalin for 3-5 years, can excellent immunostaining still be done on it. I am guessing that some sort of antigen retrieval technique can be done on the tissue, but I am not sure, since we don't do immunostaining ourselves. Thanks, Tim Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
