I'm going to try sectioning at higher temps today and I hope that it works. Thanks all for the suggestions. We used to have a vibratome but the thing only worked right for certain kinds of samples. I don't see us getting another one since the occasion to use something like that happens very infrequently. Too bad. Yes, the brains were fixed - treated with mercury and have a gray-green cast to them (wearing gloves). They were also sucrose cryoprotected.
Andi Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algra...@email.arizona.edu<mailto:algra...@email.arizona.edu> Tel: 520.626.4415 Fax: 520.626.2097 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
