Dear Mary Ann, I'll (hopefully) beat Gayle Callis to the punch on red chromogen/fluorescent detection. According to her experience, you need to slightly understain the red chromogen to achieve the best fluorescent signal. I don't know if it has to do with saturation but too much red deposition = less fluorescence.
We also use a Texas Red excitation filter on our scope. Texas Red is a fluorophore used for labeling in IHC (among other things), and the filter is one that will excite at that wavelength. It also excites the Alexa594 fluorophore which is what we use extensively. Teri Johnson Manager, Histology Genomics Institute for Novartis Research Foundation San Diego, CA 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
