also have IVD mammoglobin, clone 31A5 rabbit monoclonal, works really well for me at 1:75- if you need/prefer IVD from cancer diagnostics, the other I posted is ASR.
Joelle Weaver MAOM, HTL (ASCP) QIHC > From: mrs.abby.map...@gmail.com > Date: Wed, 22 Jan 2014 08:11:11 -0600 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Cell Marque Mammoglobin (31A5) New Protocol? > > Fellow histotechs, > > We've been running mammoglobin testing on our Ventana Benchmark XT. > However, Cell Marque (our supplier of mammoglobin antibody) has changed the > concentration. Our old concentration was 0.12 mg/mL, the new concentration > is weaker at 0.04 mg/mL. The antibody is a rabbit monoclonal. We tried > running the old protocol with the new concentration, but the results were > not adequate (see below). We tried running the protocol given to us by Cell > Marque, but theirs is specific towards the Benchmark UltraView and also > returned inadequate results. > > We process our tissue from formalin to paraffin. Our lab uses the iVew DAB > detection kit. > > Our old protocol: > Deparaffinization > Conditioner #1 (short 8 minute conditioning) > Mild CC1 (mild 30 minutes conditioning) > Standard CC1 (standard 60 minute conditioning) > No enzyme > 37C antibody incubation temperature > No titration > Antibody: Mammoglobin 31A5 at 0.12 mg/mL concentration for 32 minutes > A/B Block > Hematoxylin counterstain 4 minutes > Blueing post counterstain 4 minutes > > Does anyone else use this product from Ventana/Cell Marque? Does anyone > have a working protocol for a weaker antibody titration? > > Any help would be greatly appreciated! > > *Abby L. H. Maples, BS, MPH, HTL (ASCP)CM* > Histotechnologist, Surgical Pathology > Mercy Medical Center > 701 10th Street SE > Cedar Rapids, IA 52403 > Lab Phone: (319) 398-6827 > Cell: (319) 432-1530 > Fax: (319) 221-8767 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet