Hello, I agree anti-human CD68 might not be best for mouse tissue but there are specific mouse CD68 (the mouse homolog to human CD68) antibodies out there. FA-11 is one of my favorites. Have used both F4/80 and (rat anti-mouse CD68) on flow, frozens and FFPE. Anna, a literature search gives you published protocols. I'm looking right now at a photo of some CD68+ macs staining on mouse tissues I took from years back. Shows them nicely. anti-mouse CD68 on mouse tissue is in the literature. Ray Seattle
----- Original Message ----- From: pru...@ihctech.net To: "Anna Hughes" <anna.c.hug...@gsk.com>, histonet@lists.utsouthwestern.edu Sent: Wednesday, February 12, 2014 12:02:07 PM Subject: [Histonet] RE: Mouse F4/80 antibody Rat anti ms F4/80 is used on mouse tissue ffpe for macrophages not anti human cd68. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E. Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Anna Hughes Sent: Wednesday, February 12, 2014 9:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mouse F4/80 antibody Hi Everyone! I was wondering if any of you have a favorite CD68 macrophage antibody that you have used on mouse tissue and is especially reliable. This is a target that has been notorious in our lab and I was wondering if there was a really good one to use. Thanks in advance for your help! Anna Hughes Anna C. Hughes, BBA, BS, HTLCM anna.c.hug...@gsk.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet