I am not sure if you are talking about the Beta Amyloid antibody, but if you are: the best way to do the antigen retrieval is 3 minutes in formic acid. Willem ________________________________
Van: histonet-boun...@lists.utsouthwestern.edu namens Pirici Daniel Verzonden: di 18-2-2014 23:59 Aan: Histonet@lists.utsouthwestern.edu Onderwerp: [Histonet] Corpora amylacea are destroyed after antigen retrieval? Has anyone observed corpora amylacea on brain tissue being destroyed after antigen retrieval (for example microwaving in citrate buffer)? We are experiencing this problem, as we are interested to study these structures, and we are not sure if it is a common artifact when preparing slides for IHC... Thanks so much for your time! Daniel ________________________________________________________________________________________________ Pirici Nicolae Daniel, MD, PhD Department of Histology University of Medicine and Pharmacy Craiova Petru Rares Street 2, 200349 Craiova, Dolj Romania _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
