Hi Sarah, I've bleached out melanin using a 5% Potassium Permangenate solution. It's very quick - about 15 to 30 minutes. Wash thoroughly in running tap water. I've done IHC after bleaching with no damage to the tissue.
Hope this helps. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu <histonet-boun...@lists.utsouthwestern.edu> on behalf of sarah.dys...@stdavids.com <sarah.dys...@stdavids.com> Sent: Thursday, March 20, 2014 1:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Melanin Bleaching... So...I have never done this before. I looked it up in the good ol' bible and found a couple protocols. The pathologist wanted to use the 10% H2O2 procedure because he thought it would be more gentle. So...the slides have been sitting in the 10% solution for 24 hours now. While it is definitely working (slow as a snail...but getting lighter...), can someone please advise on what the end point of this looks like?? Thanks!! =) Sarah E. Dysart, BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor St. David's North Austin Medical Center 12221 North Mopac Expressway Austin, Texas 78758 (512)901-1220 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet