Fortunately, they say nothing at all because if that were the case, they would no longer be able to peddle their Proficiency Programs for IHC, since those too, are fixed and processed elsewhere.
Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 -----Original Message----- From: Cartun, Richard [mailto:richard.car...@hhchealth.org] Sent: Wednesday, March 26, 2014 11:47 AM To: Terri Braud; histonet@lists.utsouthwestern.edu Subject: RE: IHC antibody optimizing & validating I have not read the entire document yet. What do they say about using tissues that have been fixed and processed elsewhere? Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.car...@hhchealth.org -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Tuesday, March 25, 2014 3:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: IHC antibody optimizing & validating CAP is very clear that in order to validate a new antibody, that: "once the stain has been optimized, that for a well characterized antibody with a limited spectrum of antigenic targets, like Chromogranin or PSA, the validation can be limited. A panel of 10 positive and 10 negative neoplasms would be sufficient in this setting. For an antibody that is not well characterized and/or has a wide range of reported reactivity, a more extensive validation is necessary. The number of tissues tested should in the circumstance be large enough to determine whether the staining profile matches that previously described. An exception to the above requirements is that studies nay not be feasible for antigens such as ALK that are only seen in rare tumors." Thus sayeth CAP. And if you're like me, I am not digging through all my cases to try to come up with 30-40 neoplasms for each antibody, so I just order Tissue Micro Arrays with the neoplasms I need. 20 positive and 20 negative neoplastic tissues on one slide for easy staining and validation. The money you save on reagents to stain one little slide, more than makes up for the cost of the slide. I hope this helps. Terri L. Braud, HT(ASCP) --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet