We use Richard Allen Type 6 paraffin for both infiltration and embedding (16 years) with no adverse effects. To validate you can get the pathologist to give you samples for the same specimens you are running. IE; gallbladder 1 for patient dx and 1 for testing, uterus, appendix, any large specimen that you can spare an extra slice. Run the patient dx specimens one night, change only the paraffin in your processor and embedding center run the test samples during the day while the processor isn't in use for patient specimens. Cut and stain per usual as time permits.
-----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cameron, Elizabeth Sent: Wednesday, April 16, 2014 9:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paraffin Hello histonetters, I recently made the move back to a hospital lab after being in research, and we are currently using two different parrafins for infiltration and embedding. I would like to change the type of paraffin that we are using (I have one in mind), but I was wondering if there were any advantages to using different paraffins for the different steps and what type of validation would need to be done to make this change. We only have one processor, so a side by side comparison would be very difficult. Any thoughts on the matter would be greatly appreciated. Thank you, Liz _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
