I have been trying to get anitbodies for microglia (CD68, CD11b, IBA-1) to work using IHC and IF (regular, co-localization, even TSA amplification) on slides from frozen tissue that has been fixed in PFA and embedded in OCT with mixed crappy results. Does anyone have any suggestions or protocols?
Thanks in advance Sam Kiousis Karmanos Cancer Institute skiou...@med.wayne.edu<mailto:skiou...@med.wayne.edu> _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
