I am working on a pathology study involving two photon microscopy of pathology samples. For our study we need to be able to mark the surface aspects of our tissue with dye prior to histology processing and multiphoton imaging. Unfortunately, most of the standard tissue marking dyes we use have extraordinarily strong fluorescence in the blue/green when excited in the near UV (2 photon wavelength). This strong fluorescence then triggers the overcurrent protection on our detectors if we stray onto the surface aspect.
Are there any tissue marking dyes that are known to survive histology processing and also generate little or no blue/green fluorescence? I'm not concerned about yellow/red fluorescence (its filtered out), or attenuation without fluorescence as we don't generally image the ink directly, it just sometimes gets in the way. I've ordered a few dyes for testing based on their composition, but I'm essentially just guessing because I haven't been able to find much information. I suppose not many people need to fluorescent image inked specimens. Thanks, Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet