I am so jealous of these kids. I didn't get near a microscope until college, let alone a microtome. I hope they have fun!
Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Mon, Jul 21, 2014 at 2:19 PM, Jennifer MacDonald <jmacdon...@mtsac.edu> wrote: > T cell and B cell markers work well with human spleen. > > > > From: Patsy Ruegg <pru...@ihctech.net> > To: "Histonet@Lists. Edu" <histonet@lists.utsouthwestern.edu> > Date: 07/21/2014 11:08 AM > Subject: [Histonet] HS IHC project > Sent by: histonet-boun...@lists.utsouthwestern.edu > > > > Friends, > > I am going to help a local HS class (a Biotech class) do > a simple IHC project. This class already processes tissue, embeds, > sections and does an H&E stain. My husband fixed up an old black AO > microtome for them to use and they actually cut sections. They cannot > use human tissue there so what they have available is mouse spleen > already ffpe. I was thinking of using a rab antibody that works on ffpe > ms spleen. Ki67 comes to mind? It has been a while since I was in the > lab so I am consulting with you all to make sure a rab poly or rab mono > Ki67 will indeed stain a ffpe ms spleen? If you have evidence of this > can you tell me which ab was used successfully. If you can think of a > better rab ab to use on the ffpe ms spleen for this project let me > know. It would help if we could keep it simple for antigen retrieval. > > One of our kind vendors will be donating IHC reagents for this HS class > project. > > I hope the IHC meeting is going well in Vegas, wish I was there. > > Cheers, > Patsy > > Patsy Ruegg, HT(ASCP)QIHC > Ruegg IHC Consulting > 40864 E Arkansas Ave > Bennett, CO 80102 > H 303-644-4538 > C 720-281-5406 > prueg...@hotmail.com > pru...@ihctech.net > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet