We have found this to be a culmination of a couple of things: Bad lot of tape from vendor Drying slides before cover slipping Too much xylene drip during coverslip (dilutes the glue stuff on the tape allowing for weak adhesive)
We use Sakura cover slipping tape for more consistent quality and adjust xylene drip accordingly and make sure slides do not sit out very long at all as xylene from the stainer will evaporate (drying slides) rather quickly. Hope this helps. Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 mjo...@metropath.com On 7/25/14, 7:47 AM, "Tommy Mai" <t...@phenopath.com> wrote: >Hey everyone, > >I was wondering if anyone else has seen this occur in their labs and how >to resolve this matter. It has to do with the coverslip using the resin >tape. However more recently, I have been noticing that there is a drying >artifact that happens right as the xylene drys. It looks almost like >when water freezes and gives that frosted look. > >Thanks, >Tommy > >This e-mail message, including any attachments, is for the sole use of >the >intended recipients and may contain privileged information. Any >unauthorized >review, use, disclosure or distribution is prohibited. If you are not the >intended >recipient, please contact the sender by e-mail and destroy all copies of >the >original message, or you may call PhenoPath Laboratories, Seattle, WA >U.S.A. >at (206) 374-9000. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet