I would recommend using Kristensen's "sodium formate/formic acid" mixture. This is more gentle than formic acid alone and almost as rapid. Barry
On Tue, Aug 5, 2014 at 12:01 PM, DiCarlo, Margaret < mdica...@kaleidahealth.org> wrote: > I use 10% formic acid for large human bones but I don't think your bone > sections are that large. > > Peggy DiCarlo > Ortho Bone Lab > BGMC > 100 High St. > Buffalo, NY 14203 > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu [mailto: > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Clough, Bret > Sent: Tuesday, August 05, 2014 11:55 > To: Histonet list serv. > Subject: [Histonet] Protocol for decalcifying and processing large bone > section. > > Hi everyone, > I was hoping someone on histonet would being willing to help me by either > sharing their protocol or advising me on decalcifying and processing large > bone sections. The bone sections are from the femural head of sheep > measuring roughly 1cm x 1 1/2 cm cube. Currently I've been decalcifying > the samples in 0.5M EDTA which is taking along time to decalcify this being > the 19th day. Any advise would be greatly appreciated! > > > Sincerely, > Bret Clough > Texas A&M Health Science Center > Temple, TX. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > The Keeping You Informed section of Kaleida Health`s website features a > wealth of information, stories and pictures about our valued workforce and > the tremendous momentum our organization is experiencing. Check us out at: > www.kaleidahealth.org/kyi > > CONFIDENTIALITY NOTICE: This email transmission and any documents, files, > or previous e-mail messages attached to it are confidential and intended > solely for the use of the individual or entity to whom they are addressed. > If you are not the intended recipient, or a person responsible for > delivering it to the intended recipient, you are hereby notified that any > further review, disclosure, copying, dissemination, distribution, or use of > any of the information contained in or attached to this e-mail transmission > is strictly prohibited. If you have received this message in error, please > notify the sender immediately by e-mail, discard any paper copies, and > delete all electronic files of the message. If you are unable to contact > the sender or you are not sure as to whether you are the intended > recipient, please call Kaleida HealthÂ’s Technology Assistance Center at (716) > 859-7777. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet