Hi Bret We are using 10% Formic Acid as well and checking the decalcifying end-point by using the faxitron x-ray system. I work with bone cancer for 99% of the time and Formic Acid is safer than any other one.
Thomas -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Wednesday, August 06, 2014 10:13 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 129, Issue 6 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Protocol for decalcifying and processing large bone section. (DiCarlo, Margaret) 2. AW: [Histonet] RE: whole slide scanners (Gudrun Lang) 3. Glassware Cleaning, CAP Checklist Item GEN.41770 (Laurie Colbert) 4. RE: RE: whole slide scanners (Elizabeth Chlipala) 5. Listservers in microbiology (Jorge A. Santiago-Blay) 6. AW: [Histonet] RE: whole slide scanners (Gudrun Lang) 7. Procedures and Policy manual needed (Dr. Max Mirot) 8. RE: Glassware Cleaning, CAP Checklist Item GEN.41770 (Cooper, Brian) 9. RE: RE: whole slide scanners (Morken, Timothy) 10. RE: Listservers in microbiology (Cartun, Richard) 11. RE: RE: whole slide scanners (James Watson) 12. RE: RE: Glassware Cleaning, CAP Checklist Item GEN.41770 (Joelle Weaver) 13. RE: RE: Glassware Cleaning, CAP Checklist Item GEN.41770 (Jamal) 14. Re: Protocol for decalcifying and processing large bone section. (Barry Rittman) 15. Re: RE: Glassware Cleaning, CAP Checklist Item GEN.41770 (Michael Ann Jones) 16. RI company seeks plastics company for research work (Hans B Snyder) 17. p16 (sarah.dys...@stdavids.com) 18. neutralize formalin (Mary Ann Deathridge) ---------------------------------------------------------------------- Message: 1 Date: Tue, 5 Aug 2014 17:01:16 +0000 From: "DiCarlo, Margaret" <mdica...@kaleidahealth.org> Subject: RE: [Histonet] Protocol for decalcifying and processing large bone section. To: "'Clough, Bret'" <clo...@medicine.tamhsc.edu>, Histonet list serv. <histonet@lists.utsouthwestern.edu> Message-ID: <731be09cdb19aa43aa8682199d42d31b1dc79...@adcexchange01.kaleidahealth.org> Content-Type: text/plain; charset="us-ascii" I use 10% formic acid for large human bones but I don't think your bone sections are that large. Peggy DiCarlo Ortho Bone Lab BGMC 100 High St. Buffalo, NY 14203 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Clough, Bret Sent: Tuesday, August 05, 2014 11:55 To: Histonet list serv. Subject: [Histonet] Protocol for decalcifying and processing large bone section. Hi everyone, I was hoping someone on histonet would being willing to help me by either sharing their protocol or advising me on decalcifying and processing large bone sections. The bone sections are from the femural head of sheep measuring roughly 1cm x 1 1/2 cm cube. Currently I've been decalcifying the samples in 0.5M EDTA which is taking along time to decalcify this being the 19th day. Any advise would be greatly appreciated! Sincerely, Bret Clough Texas A&M Health Science Center Temple, TX. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The Keeping You Informed section of Kaleida Health`s website features a wealth of information, stories and pictures about our valued workforce and the tremendous momentum our organization is experiencing. Check us out at: www.kaleidahealth.org/kyi CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please call Kaleida Health??s Technology Assistance Center at (716) 859-7777. ------------------------------ Message: 2 Date: Tue, 5 Aug 2014 19:12:42 +0200 From: "Gudrun Lang" <gu.l...@gmx.at> Subject: AW: [Histonet] RE: whole slide scanners To: "'Elizabeth Chlipala'" <l...@premierlab.com> Cc: histonet@lists.utsouthwestern.edu Message-ID: <000a01cfb0d0$7948bd10$6bda3730$@gmx.at> Content-Type: text/plain; charset="iso-8859-1" Hi, for which purpose do you use the high troughput scanner. Archive or diagnostics? Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Elizabeth Chlipala Gesendet: Dienstag, 05. August 2014 17:16 An: James Watson; 'Kalleberg, Kristopher'; histonet-requ...@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Betreff: [Histonet] RE: whole slide scanners I agree with James the type of scanner you need will be dependent upon your use cases and workload. We have an Aperio ScanScope XT which is an 120 slide scanner and that works well for us. I'm going to make a shameless plug for a workshop that myself, Bill DeSalvo and Jesus Ellin will be giving at the annual meeting in Austin this year, it's called Digital Pathology for the Histotech - A Guide to Implementation it's an all day workshop that is on Saturday the 23rd and it will cover all aspects of how to select a scanner, how to implement scanning, etc. It will be very valuable to histotechs that are already scanning and also techs that are new to the scanning process. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of James Watson Sent: Tuesday, August 05, 2014 8:14 AM To: 'Kalleberg, Kristopher'; histonet-requ...@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: whole slide scanners The scanner you get depends on your usage and volume. Three that I recommend are for high volume. Aperio, Good image quality, if you need to link to study metadata Spectrum software is great. Recommend for chromogen. Separate scanner for fluorescence, fluorescent scanner only holds 5 slides at a time. Hamamatsu Nanozoomer, What we use, we do high volume chromogen and fluorescence, excellent for both. Our NanoZoomer holds 210 slides for a chromogen run. Can load up about 100 fluorescent slides per night, auto focus works well. Philips, Chromogen only, very fast scanning with high quality scans. 60 slides per hour. There are many others on the market for smaller workloads. James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel??? 858-332-4647 Fax?? 858-812-1915 jwat...@gnf.org -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kalleberg, Kristopher Sent: Tuesday, August 05, 2014 5:18 AM To: histonet-requ...@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] whole slide scanners Hello All, I am looking into the purchase of a whole slide scanner. If anyone could supply some recommendations it would be greatly appreciated. Thank you. Kris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Tue, 5 Aug 2014 18:05:49 +0000 From: Laurie Colbert <lcolb...@pathmdlabs.com> Subject: [Histonet] Glassware Cleaning, CAP Checklist Item GEN.41770 To: "Histonet Post (histonet@lists.utsouthwestern.edu)" <histonet@lists.utsouthwestern.edu> Message-ID: <12ECD7346266D74691EC2BFC75285E452F4B1C6D@BFL323E10.pathmdlabs.local> Content-Type: text/plain; charset="us-ascii" Do others in Histology follow these CAP Guidelines for cleaning glassware? I'm not sure if this question actually applies to Histology. I have never had an inspector ask me about it, but I'm wondering if anyone has been questioned. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. ------------------------------ Message: 4 Date: Tue, 5 Aug 2014 12:21:00 -0600 From: Elizabeth Chlipala <l...@premierlab.com> Subject: RE: [Histonet] RE: whole slide scanners To: Gudrun Lang <gu.l...@gmx.at> Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <14E2C6176416974295479C64A11CB9AE019C79ECD460@SBS2K8.premierlab.local> Content-Type: text/plain; charset="iso-8859-1" We are a research based histology lab so we use scanning for several reasons: 1. We scan slides for clients so they can review their images remotely and capture snapshot images, annotate, etc- study sizes may range from 10 to 100's of slides 2. We scan all of our slides for IHC protocol development - our clients that request us to develop IHC staining protocols we scan slides so they can also view the slides and track our progress, plus this creates a great way for us to review the results too, we can easily determine the correct dilution by viewing multiple dilutions at the same time. 3. We scan all of our stained slides from the control blocks and these are linked to our control block database so anyone can view the scanned slide to see if the controls are working properly. 4. We have studies that require image analysis so all of the slides are scanned and then analyzed via a custom algorithm that will generate the data required for a particular study design. 5. We scan all of our IHC control slides so we can tract staining consistency These are just a few of the applications that we use WSI for. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Gudrun Lang [mailto:gu.l...@gmx.at] Sent: Tuesday, August 05, 2014 11:13 AM To: Elizabeth Chlipala Cc: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] RE: whole slide scanners Hi, for which purpose do you use the high troughput scanner. Archive or diagnostics? Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Elizabeth Chlipala Gesendet: Dienstag, 05. August 2014 17:16 An: James Watson; 'Kalleberg, Kristopher'; histonet-requ...@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Betreff: [Histonet] RE: whole slide scanners I agree with James the type of scanner you need will be dependent upon your use cases and workload. We have an Aperio ScanScope XT which is an 120 slide scanner and that works well for us. I'm going to make a shameless plug for a workshop that myself, Bill DeSalvo and Jesus Ellin will be giving at the annual meeting in Austin this year, it's called Digital Pathology for the Histotech - A Guide to Implementation it's an all day workshop that is on Saturday the 23rd and it will cover all aspects of how to select a scanner, how to implement scanning, etc. It will be very valuable to histotechs that are already scanning and also techs that are new to the scanning process. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of James Watson Sent: Tuesday, August 05, 2014 8:14 AM To: 'Kalleberg, Kristopher'; histonet-requ...@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: whole slide scanners The scanner you get depends on your usage and volume. Three that I recommend are for high volume. Aperio, Good image quality, if you need to link to study metadata Spectrum software is great. Recommend for chromogen. Separate scanner for fluorescence, fluorescent scanner only holds 5 slides at a time. Hamamatsu Nanozoomer, What we use, we do high volume chromogen and fluorescence, excellent for both. Our NanoZoomer holds 210 slides for a chromogen run. Can load up about 100 fluorescent slides per night, auto focus works well. Philips, Chromogen only, very fast scanning with high quality scans. 60 slides per hour. There are many others on the market for smaller workloads. James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel??? 858-332-4647 Fax?? 858-812-1915 jwat...@gnf.org -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kalleberg, Kristopher Sent: Tuesday, August 05, 2014 5:18 AM To: histonet-requ...@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] whole slide scanners Hello All, I am looking into the purchase of a whole slide scanner. If anyone could supply some recommendations it would be greatly appreciated. Thank you. Kris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Tue, 5 Aug 2014 14:39:18 -0400 From: "Jorge A. Santiago-Blay" <blayjo...@gmail.com> Subject: [Histonet] Listservers in microbiology To: histonet@lists.utsouthwestern.edu Message-ID: <cagbdduduoo32hd3cbsew_b3pzxvdh3f_xvpkguqyq1mo45-...@mail.gmail.com> Content-Type: text/plain; charset=UTF-8 Hello: I have found Histonet quite useful and the members with whom I have communicated helpful. Can someone recommend listservers with similar qualities that emphasize microbiology? If "yes", please feel free to email at blayjo...@gmail.com Gratefully, Jorge Jorge A. Santiago-Blay, PhD blaypublishers.com http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.html ------------------------------ Message: 6 Date: Tue, 5 Aug 2014 20:54:07 +0200 From: "Gudrun Lang" <gu.l...@gmx.at> Subject: AW: [Histonet] RE: whole slide scanners To: "'Gudrun Lang'" <gu.l...@gmx.at> Cc: Histonet@lists.utsouthwestern.edu Message-ID: <000a01cfb0de$a31e6460$e95b2d20$@gmx.at> Content-Type: text/plain; charset="iso-8859-1" Does anyone out there use digital pathology for routine diagnostics? Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Gudrun Lang Gesendet: Dienstag, 05. August 2014 19:13 An: 'Elizabeth Chlipala' Cc: histonet@lists.utsouthwestern.edu Betreff: AW: [Histonet] RE: whole slide scanners Hi, for which purpose do you use the high troughput scanner. Archive or diagnostics? Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Elizabeth Chlipala Gesendet: Dienstag, 05. August 2014 17:16 An: James Watson; 'Kalleberg, Kristopher'; histonet-requ...@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Betreff: [Histonet] RE: whole slide scanners I agree with James the type of scanner you need will be dependent upon your use cases and workload. We have an Aperio ScanScope XT which is an 120 slide scanner and that works well for us. I'm going to make a shameless plug for a workshop that myself, Bill DeSalvo and Jesus Ellin will be giving at the annual meeting in Austin this year, it's called Digital Pathology for the Histotech - A Guide to Implementation it's an all day workshop that is on Saturday the 23rd and it will cover all aspects of how to select a scanner, how to implement scanning, etc. It will be very valuable to histotechs that are already scanning and also techs that are new to the scanning process. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of James Watson Sent: Tuesday, August 05, 2014 8:14 AM To: 'Kalleberg, Kristopher'; histonet-requ...@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: whole slide scanners The scanner you get depends on your usage and volume. Three that I recommend are for high volume. Aperio, Good image quality, if you need to link to study metadata Spectrum software is great. Recommend for chromogen. Separate scanner for fluorescence, fluorescent scanner only holds 5 slides at a time. Hamamatsu Nanozoomer, What we use, we do high volume chromogen and fluorescence, excellent for both. Our NanoZoomer holds 210 slides for a chromogen run. Can load up about 100 fluorescent slides per night, auto focus works well. Philips, Chromogen only, very fast scanning with high quality scans. 60 slides per hour. There are many others on the market for smaller workloads. James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel??? 858-332-4647 Fax?? 858-812-1915 jwat...@gnf.org -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kalleberg, Kristopher Sent: Tuesday, August 05, 2014 5:18 AM To: histonet-requ...@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] whole slide scanners Hello All, I am looking into the purchase of a whole slide scanner. If anyone could supply some recommendations it would be greatly appreciated. Thank you. Kris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Tue, 5 Aug 2014 18:57:54 +0000 From: "Dr. Max Mirot" <max.mi...@svrhc.org> Subject: [Histonet] Procedures and Policy manual needed To: "'histonet@lists.utsouthwestern.edu'" <histonet@lists.utsouthwestern.edu> Message-ID: <e7d668c2c687134b9fae3268d55741ac023...@svexch05.svrhc.org> Content-Type: text/plain; charset="us-ascii" I am attempting to re-write our procedure and policy manual. Our scope of service is routine anatomic pathology/histology and Non Gyn cytology. Right now the lab is CLIA certified. Anyone care to share their policy and procedure manual? I would like to look at a couple of nicely done manuals to serve as template. It can be for CAP not just CLIA. Send off list to me max.mi...@svrhc.org<mailto:max.mi...@svrhc.org> Thanks Max S. Mirot, M.D. ------------------------------ Message: 8 Date: Tue, 5 Aug 2014 19:07:05 +0000 From: "Cooper, Brian" <bcoo...@chla.usc.edu> Subject: [Histonet] RE: Glassware Cleaning, CAP Checklist Item GEN.41770 To: "Laurie Colbert" <lcolb...@pathmdlabs.com>, "Histonet Post (histonet@lists.utsouthwestern.edu)" <histonet@lists.utsouthwestern.edu> Message-ID: <c12647ad4408834c8ab48fb0389c63e331402...@chlaexmbh01.la.ad.chla.org> Content-Type: text/plain; charset=iso-8859-1 I've never been asked by a CAP auditor either, but our internal inspectors used to inquire about this in a previous lab all the time. We use Bromocresol Purple as an indicator here, and we have a simple log sheet wherein our crew just tests one piece of cleaned glassware each day. They write the type of glassware they test, and place a checkmark in a "yellow" or "purple" column based upon what they see. If "purple," they rewash, retest and document accordingly. Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357???? Pager: 213-209-0184 bcoo...@chla.usc.edu -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Tuesday, August 05, 2014 11:06 AM To: Histonet Post (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Glassware Cleaning, CAP Checklist Item GEN.41770 Do others in Histology follow these CAP Guidelines for cleaning glassware? I'm not sure if this question actually applies to Histology. I have never had an inspector ask me about it, but I'm wondering if anyone has been questioned. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- ------------------------------ Message: 9 Date: Tue, 5 Aug 2014 19:07:50 +0000 From: "Morken, Timothy" <timothy.mor...@ucsfmedctr.org> Subject: RE: [Histonet] RE: whole slide scanners To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <761e2b5697f795489c8710bcc72141ff36790...@ex07.net.ucsf.edu> Content-Type: text/plain; charset=iso-8859-1 We are setting up Philips high speed slide scanners to scan frozen sections for remote Dx. One critical aspect is to get coverslip medium that dries fast... Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Tuesday, August 05, 2014 10:13 AM To: 'Elizabeth Chlipala' Cc: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] RE: whole slide scanners Hi, for which purpose do you use the high troughput scanner. Archive or diagnostics? Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Elizabeth Chlipala Gesendet: Dienstag, 05. August 2014 17:16 An: James Watson; 'Kalleberg, Kristopher'; histonet-requ...@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Betreff: [Histonet] RE: whole slide scanners I agree with James the type of scanner you need will be dependent upon your use cases and workload. We have an Aperio ScanScope XT which is an 120 slide scanner and that works well for us. I'm going to make a shameless plug for a workshop that myself, Bill DeSalvo and Jesus Ellin will be giving at the annual meeting in Austin this year, it's called Digital Pathology for the Histotech - A Guide to Implementation it's an all day workshop that is on Saturday the 23rd and it will cover all aspects of how to select a scanner, how to implement scanning, etc. It will be very valuable to histotechs that are already scanning and also techs that are new to the scanning process. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of James Watson Sent: Tuesday, August 05, 2014 8:14 AM To: 'Kalleberg, Kristopher'; histonet-requ...@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: whole slide scanners The scanner you get depends on your usage and volume. Three that I recommend are for high volume. Aperio, Good image quality, if you need to link to study metadata Spectrum software is great. Recommend for chromogen. Separate scanner for fluorescence, fluorescent scanner only holds 5 slides at a time. Hamamatsu Nanozoomer, What we use, we do high volume chromogen and fluorescence, excellent for both. Our NanoZoomer holds 210 slides for a chromogen run. Can load up about 100 fluorescent slides per night, auto focus works well. Philips, Chromogen only, very fast scanning with high quality scans. 60 slides per hour. There are many others on the market for smaller workloads. James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel??? 858-332-4647 Fax?? 858-812-1915 jwat...@gnf.org -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kalleberg, Kristopher Sent: Tuesday, August 05, 2014 5:18 AM To: histonet-requ...@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] whole slide scanners Hello All, I am looking into the purchase of a whole slide scanner. If anyone could supply some recommendations it would be greatly appreciated. Thank you. Kris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Tue, 5 Aug 2014 19:15:46 +0000 From: "Cartun, Richard" <richard.car...@hhchealth.org> Subject: RE: [Histonet] Listservers in microbiology To: "Jorge A. Santiago-Blay" <blayjo...@gmail.com>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <9215bd4b0ba1b44d962a71c758b68d2e355c2...@hhcexchmb03.hhcsystem.org> Content-Type: text/plain; charset="utf-8" Our Director of Microbiology gave me this information: "Yes there is an excellent Listserver in microbiology but it is by invitation and requires ASM membership. Clinmicronetgroup." Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.car...@hhchealth.org -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jorge A. Santiago-Blay Sent: Tuesday, August 05, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Listservers in microbiology Hello: I have found Histonet quite useful and the members with whom I have communicated helpful. Can someone recommend listservers with similar qualities that emphasize microbiology? If "yes", please feel free to email at blayjo...@gmail.com Gratefully, Jorge Jorge A. Santiago-Blay, PhD blaypublishers.com http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ------------------------------ Message: 11 Date: Tue, 5 Aug 2014 19:30:32 +0000 From: James Watson <jwat...@gnf.org> Subject: RE: [Histonet] RE: whole slide scanners To: 'Gudrun Lang' <gu.l...@gmx.at>, 'Elizabeth Chlipala' <l...@premierlab.com> Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <a032b25305ec6949ac9aff23c9620616c9f3b...@ex5.lj.gnf.org> Content-Type: text/plain; charset="iso-8859-1" We are pharma research, about 40-45% of our slides are fluorescent. With 1 scanner. 2013 17,096 slides scanned 2013 20,501 slides scanned James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel??? 858-332-4647 Fax?? 858-812-1915 jwat...@gnf.org -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Tuesday, August 05, 2014 10:13 AM To: 'Elizabeth Chlipala' Cc: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] RE: whole slide scanners Hi, for which purpose do you use the high troughput scanner. Archive or diagnostics? Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Elizabeth Chlipala Gesendet: Dienstag, 05. August 2014 17:16 An: James Watson; 'Kalleberg, Kristopher'; histonet-requ...@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Betreff: [Histonet] RE: whole slide scanners I agree with James the type of scanner you need will be dependent upon your use cases and workload. We have an Aperio ScanScope XT which is an 120 slide scanner and that works well for us. I'm going to make a shameless plug for a workshop that myself, Bill DeSalvo and Jesus Ellin will be giving at the annual meeting in Austin this year, it's called Digital Pathology for the Histotech - A Guide to Implementation it's an all day workshop that is on Saturday the 23rd and it will cover all aspects of how to select a scanner, how to implement scanning, etc. It will be very valuable to histotechs that are already scanning and also techs that are new to the scanning process. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of James Watson Sent: Tuesday, August 05, 2014 8:14 AM To: 'Kalleberg, Kristopher'; histonet-requ...@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: whole slide scanners The scanner you get depends on your usage and volume. Three that I recommend are for high volume. Aperio, Good image quality, if you need to link to study metadata Spectrum software is great. Recommend for chromogen. Separate scanner for fluorescence, fluorescent scanner only holds 5 slides at a time. Hamamatsu Nanozoomer, What we use, we do high volume chromogen and fluorescence, excellent for both. Our NanoZoomer holds 210 slides for a chromogen run. Can load up about 100 fluorescent slides per night, auto focus works well. Philips, Chromogen only, very fast scanning with high quality scans. 60 slides per hour. There are many others on the market for smaller workloads. James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel??? 858-332-4647 Fax?? 858-812-1915 jwat...@gnf.org -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kalleberg, Kristopher Sent: Tuesday, August 05, 2014 5:18 AM To: histonet-requ...@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] whole slide scanners Hello All, I am looking into the purchase of a whole slide scanner. If anyone could supply some recommendations it would be greatly appreciated. Thank you. Kris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Tue, 5 Aug 2014 19:33:20 +0000 From: Joelle Weaver <joellewea...@hotmail.com> Subject: RE: [Histonet] RE: Glassware Cleaning, CAP Checklist Item GEN.41770 To: "Cooper, Brian" <bcoo...@chla.usc.edu>, Laurie Colbert <lcolb...@pathmdlabs.com>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <snt149-w34211a5685aad1df896006d8...@phx.gbl> Content-Type: text/plain; charset="iso-8859-1" Do the same as Brian. It is not that involved really, and its not worth getting a deficiency over. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: bcoo...@chla.usc.edu > To: lcolb...@pathmdlabs.com; histonet@lists.utsouthwestern.edu > Date: Tue, 5 Aug 2014 19:07:05 +0000 > CC: > Subject: [Histonet] RE: Glassware Cleaning, CAP Checklist Item GEN.41770 > > I've never been asked by a CAP auditor either, but our internal inspectors > used to inquire about this in a previous lab all the time. We use > Bromocresol Purple as an indicator here, and we have a simple log sheet > wherein our crew just tests one piece of cleaned glassware each day. They > write the type of glassware they test, and place a checkmark in a "yellow" or > "purple" column based upon what they see. If "purple," they rewash, retest > and document accordingly. > > Brian D. Cooper, HT (ASCP)CM | Histology Supervisor > Department of Pathology and Laboratory Medicine > Children's Hospital Los Angeles > 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 > Ph: 323.361.3357 Pager: 213-209-0184 > bcoo...@chla.usc.edu > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert > Sent: Tuesday, August 05, 2014 11:06 AM > To: Histonet Post (histonet@lists.utsouthwestern.edu) > Subject: [Histonet] Glassware Cleaning, CAP Checklist Item GEN.41770 > > Do others in Histology follow these CAP Guidelines for cleaning glassware? > I'm not sure if this question actually applies to Histology. I have never > had an inspector ask me about it, but I'm wondering if anyone has been > questioned. > > Laurie Colbert, HT (ASCP) > Histology Supervisor > PATH MD > 8158 Beverly Blvd. > Los Angeles, CA 90048 > (323) 648-3214 direct > (424) 245-7284 main lab > > The information contained in this transmission may contain privileged and > confidential information, including patient information protected by federal > and state privacy laws. It is intended only for the use of the person(s) > named above. If you are not the intended recipient, you are hereby notified > that any review, dissemination, distribution, or duplication of this > communication is strictly prohibited. If you are not the intended recipient, > please contact the sender by reply email and destroy all copies of the > original message. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------------------------------------------- > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, > is for the sole use of the intended recipient(s) and may contain confidential > or legally privileged information. Any unauthorized review, use, disclosure > or distribution is prohibited. If you are not the intended recipient, please > contact the sender by reply e-mail and destroy all copies of this original > message. > > --------------------------------------------------------------------- > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Wed, 6 Aug 2014 11:25:48 +0300 From: "Jamal" <j.rowa...@alborglaboratories.com> Subject: RE: [Histonet] RE: Glassware Cleaning, CAP Checklist Item GEN.41770 To: "'Joelle Weaver'" <joellewea...@hotmail.com>, "'Cooper, Brian'" <bcoo...@chla.usc.edu>, "'Laurie Colbert'" <lcolb...@pathmdlabs.com>, <histonet@lists.utsouthwestern.edu> Message-ID: <01f901cfb150$0864d680$192e8380$@rowa...@alborglaboratories.com> Content-Type: text/plain; charset="windows-1256" I am also the same as Joelle & Brian, It never been questioned by CAP inspectors but since it's in the common checklist we have to follow that?s why we have a policy with simple procedure and record, the procedure almost the same as Brian procedure but some time we use the digital PH meter. Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories |? Mobile +966 503629832| j.rowa...@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joelle Weaver Sent: Tuesday, August 05, 2014 10:33 PM To: Cooper, Brian; Laurie Colbert; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Glassware Cleaning, CAP Checklist Item GEN.41770 Do the same as Brian. It is not that involved really, and its not worth getting a deficiency over. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: bcoo...@chla.usc.edu > To: lcolb...@pathmdlabs.com; histonet@lists.utsouthwestern.edu > Date: Tue, 5 Aug 2014 19:07:05 +0000 > CC: > Subject: [Histonet] RE: Glassware Cleaning, CAP Checklist Item GEN.41770 > > I've never been asked by a CAP auditor either, but our internal inspectors used to inquire about this in a previous lab all the time. We use Bromocresol Purple as an indicator here, and we have a simple log sheet wherein our crew just tests one piece of cleaned glassware each day. They write the type of glassware they test, and place a checkmark in a "yellow" or "purple" column based upon what they see. If "purple," they rewash, retest and document accordingly. > > Brian D. Cooper, HT (ASCP)CM | Histology Supervisor > Department of Pathology and Laboratory Medicine > Children's Hospital Los Angeles > 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 > Ph: 323.361.3357 Pager: 213-209-0184 > bcoo...@chla.usc.edu > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert > Sent: Tuesday, August 05, 2014 11:06 AM > To: Histonet Post (histonet@lists.utsouthwestern.edu) > Subject: [Histonet] Glassware Cleaning, CAP Checklist Item GEN.41770 > > Do others in Histology follow these CAP Guidelines for cleaning glassware? I'm not sure if this question actually applies to Histology. I have never had an inspector ask me about it, but I'm wondering if anyone has been questioned. > > Laurie Colbert, HT (ASCP) > Histology Supervisor > PATH MD > 8158 Beverly Blvd. > Los Angeles, CA 90048 > (323) 648-3214 direct > (424) 245-7284 main lab > > The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------------------------------------------- > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, > is for the sole use of the intended recipient(s) and may contain confidential > or legally privileged information. Any unauthorized review, use, disclosure > or distribution is prohibited. If you are not the intended recipient, please > contact the sender by reply e-mail and destroy all copies of this original message. > > --------------------------------------------------------------------- > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Wed, 6 Aug 2014 07:02:10 -0500 From: Barry Rittman <barryritt...@gmail.com> Subject: Re: [Histonet] Protocol for decalcifying and processing large bone section. To: "Histonet list serv." <histonet@lists.utsouthwestern.edu> Message-ID: <CA+0tsF0Oqy9u3rVVuSiT5gJRKDdzkmTrVM7u-it4d8jnGq=-m...@mail.gmail.com> Content-Type: text/plain; charset=UTF-8 I would recommend using Kristensen's "sodium formate/formic acid" mixture. This is more gentle than formic acid alone and almost as rapid. Barry On Tue, Aug 5, 2014 at 12:01 PM, DiCarlo, Margaret < mdica...@kaleidahealth.org> wrote: > I use 10% formic acid for large human bones but I don't think your bone > sections are that large. > > Peggy DiCarlo > Ortho Bone Lab > BGMC > 100 High St. > Buffalo, NY 14203 > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu [mailto: > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Clough, Bret > Sent: Tuesday, August 05, 2014 11:55 > To: Histonet list serv. > Subject: [Histonet] Protocol for decalcifying and processing large bone > section. > > Hi everyone, > I was hoping someone on histonet would being willing to help me by either > sharing their protocol or advising me on decalcifying and processing large > bone sections. The bone sections are from the femural head of sheep > measuring roughly 1cm x 1 1/2 cm cube. Currently I've been decalcifying > the samples in 0.5M EDTA which is taking along time to decalcify this being > the 19th day. Any advise would be greatly appreciated! > > > Sincerely, > Bret Clough > Texas A&M Health Science Center > Temple, TX. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > The Keeping You Informed section of Kaleida Health`s website features a > wealth of information, stories and pictures about our valued workforce and > the tremendous momentum our organization is experiencing. Check us out at: > www.kaleidahealth.org/kyi > > CONFIDENTIALITY NOTICE: This email transmission and any documents, files, > or previous e-mail messages attached to it are confidential and intended > solely for the use of the individual or entity to whom they are addressed. > If you are not the intended recipient, or a person responsible for > delivering it to the intended recipient, you are hereby notified that any > further review, disclosure, copying, dissemination, distribution, or use of > any of the information contained in or attached to this e-mail transmission > is strictly prohibited. If you have received this message in error, please > notify the sender immediately by e-mail, discard any paper copies, and > delete all electronic files of the message. If you are unable to contact > the sender or you are not sure as to whether you are the intended > recipient, please call Kaleida Health?????s Technology Assistance Center at > (716) > 859-7777. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 15 Date: Wed, 6 Aug 2014 13:12:58 +0000 From: Michael Ann Jones <mjo...@metropath.com> Subject: Re: [Histonet] RE: Glassware Cleaning, CAP Checklist Item GEN.41770 To: "Cooper, Brian" <bcoo...@chla.usc.edu>, Laurie Colbert <lcolb...@pathmdlabs.com>, "Histonet Post (histonet@lists.utsouthwestern.edu)" <histonet@lists.utsouthwestern.edu> Message-ID: <d0078712.2433%mjo...@metropath.com> Content-Type: text/plain; charset="us-ascii" Ditto with Brian Cooper. Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 mjo...@metropath.com On 8/5/14, 1:07 PM, "Cooper, Brian" <bcoo...@chla.usc.edu> wrote: >I've never been asked by a CAP auditor either, but our internal >inspectors used to inquire about this in a previous lab all the time. We >use Bromocresol Purple as an indicator here, and we have a simple log >sheet wherein our crew just tests one piece of cleaned glassware each >day. They write the type of glassware they test, and place a checkmark >in a "yellow" or "purple" column based upon what they see. If "purple," >they rewash, retest and document accordingly. > >Brian D. Cooper, HT (ASCP)CM | Histology Supervisor >Department of Pathology and Laboratory Medicine >Children's Hospital Los Angeles >4650 Sunset Blvd MS#43- Los Angeles, CA 90027 >Ph: 323.361.3357 Pager: 213-209-0184 >bcoo...@chla.usc.edu > >-----Original Message----- >From: histonet-boun...@lists.utsouthwestern.edu >[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie >Colbert >Sent: Tuesday, August 05, 2014 11:06 AM >To: Histonet Post (histonet@lists.utsouthwestern.edu) >Subject: [Histonet] Glassware Cleaning, CAP Checklist Item GEN.41770 > >Do others in Histology follow these CAP Guidelines for cleaning >glassware? I'm not sure if this question actually applies to Histology. > I have never had an inspector ask me about it, but I'm wondering if >anyone has been questioned. > >Laurie Colbert, HT (ASCP) >Histology Supervisor >PATH MD >8158 Beverly Blvd. >Los Angeles, CA 90048 >(323) 648-3214 direct >(424) 245-7284 main lab > >The information contained in this transmission may contain privileged and >confidential information, including patient information protected by >federal and state privacy laws. It is intended only for the use of the >person(s) named above. If you are not the intended recipient, you are >hereby notified that any review, dissemination, distribution, or >duplication of this communication is strictly prohibited. If you are not >the intended recipient, please contact the sender by reply email and >destroy all copies of the original message. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >--------------------------------------------------------------------- >CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, >is for the sole use of the intended recipient(s) and may contain >confidential >or legally privileged information. Any unauthorized review, use, >disclosure >or distribution is prohibited. If you are not the intended recipient, >please >contact the sender by reply e-mail and destroy all copies of this >original message. > >--------------------------------------------------------------------- > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Wed, 6 Aug 2014 09:50:03 -0400 From: Hans B Snyder <h...@histologistics.com> Subject: [Histonet] RI company seeks plastics company for research work To: "histonet@lists.utsouthwestern.edu" <Histonet@lists.utsouthwestern.edu> Message-ID: <CAAYBjcsn1tH1zTvDMSKQt74AGV7J3Nub-fJaxV=99juleta...@mail.gmail.com> Content-Type: text/plain; charset=UTF-8 Hello All, I have a company who needs: "a source of basic services for sectioning of GMA blocks containing biomaterial devices and subsequent H&E staining. The devices are cut for embedding to about 8mm in length, and contain adhesives, thermoplastic membranes, various scaffolds, and cells. Our group would do the embedding and transfer samples to you, and would be looking for about 25-50 samples/month." I would like to give him references so that he can email and or call places he chooses. Please email me and I will forward all information. Thank you Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 h...@histologistics.com ------------------------------ Message: 17 Date: Wed, 6 Aug 2014 09:51:09 -0500 From: <sarah.dys...@stdavids.com> Subject: [Histonet] p16 To: <histonet@lists.utsouthwestern.edu> Message-ID: <34c22bb94729434598d767d3f4eb95e0cfa038e...@fwdcwpmsgcms03.hca.corpad.net> Content-Type: text/plain; charset="us-ascii" Does anyone know of a comparable clone to the Ventana p16 clone E6H4? I would prefer it to be undiluted (concentrate) so that we can titrate out the antibody ourselves. We need it to have publications that would back this up and/or stained slide pictures I can show our pathologist. Thanks Sarah E. Dysart, BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor St. David's North Austin Medical Center 12221 North Mopac Expressway Austin, Texas 78758 (512)901-1220 ------------------------------ Message: 18 Date: Wed, 6 Aug 2014 08:06:42 -0700 From: "Mary Ann Deathridge" <madeathri...@pastnashville.com> Subject: [Histonet] neutralize formalin To: <histonet@lists.utsouthwestern.edu> Message-ID: <eccb9838e4bc4e718999081d3a1da...@pastnashville.com> Content-Type: text/plain; charset="us-ascii" We pour our waste xylene/alcohol into a drum and the formalin waste in a seperate drum for a waste service company to remove. Problem is space to house 55 gal drums and we have more xylene/alc waste than formalin. Does anyone neutralize formalin and dispose of it down the drain? If so can you recommend the method/product utilized. Xylene recyler is not an option. Maryann Deathridge, BS, HT (ASCP) Lab Manager 615-298-4100, Fax: 615-298-4141 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 129, Issue 6 **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet