Hi Gayle, I do get your view on making a judgement. We do our own issues at times, in microtomy/staining. Nicely elaborated.I would like to go through the publication to have a better understanding. Pl. do share to my email- ragh...@orchidpharma.com.
Thanks, raghul -----Original Message----- From: gayle callis [mailto:gayle.cal...@bresnan.net] Sent: 13 August 2014 20:12 To: Raghul J (Biology); histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Weight Loss/Weight Gain Decal _Histonet Digest, Vol 129, Issue 18 The best way to determine the final endpoint is radiography as it is the most sensitive if you have a FAXITRON. If you have this xray machine or a microCT, then you should use these for decalcification endpoint and not waste time with weight loss/weight gain method or any other method. When we do the weight loss/weight gain, we simply made the judgement call that the weight gain means no calcium is present and no further testing is required. It is considered bad practice to "prick" the bone as you may 1) create needle tract artifact into critical areas you might want to see 2) possibly dislodge a small pathological lesion from the bone 3) bending bone can dislodge tumor from bone . Consequently, we never did mechanical testing EVER! We would decalcify as many as 40 to 50 samples - a collection of NBF fixed mouse knees, or tibias, or paws at one time in a large 1 to 2 liters 10% to 15% formic acid. Having to weigh each and every one of these small bones was tedious and time consuming. We found that if we took representative samples of each kind of bone - 4 from experimental group, and 4 from control group and use these bones as the wt loss/wt gain samples, the remaining samples still decalcifying were so close in size (and weight), we had excellent decalcification without damaging the bone. One learns very quickly which murine leg bones decalcify faster than other with paws taking much more time due to the tiny bones so tightly packed together. The original Mawhinney et al weight gain/weight loss publication tested the last nitric acid change with a chemical test. Unfortunately, doing chemical testing with EDTA is even more tedious. Some people will actually let the bones sit a few hours longer in formic acid decalcifiers to ensure the calcium is removed but you may over expose and do acid hydrolysis the antigens, nuclei i.e. "overdecalcification" . You can do this but I suggest testing the bones again so see if that weight gain has increased or not. If not, then you may have left the bones in acid too long. It doesn't take long to damage staining with acid exposure. A chemical endpoint test is more accurate than weight loss/weight gain, but you can't be doing a huge number of samples in one container of decalcifying solution. This is probably the reason those authors did chemical test after weight loss/weight gain but with single samples. I know of one person who success doing chemical test for 4 to 5 bone slabs of same size and approximate weight, decalcfified in an acid solution, and did the chemical test from a single container of used, never stirred decalcifier for those 4 or 5 large samples. I will be happy to send the original publication to you via private email, and the chemical test method. Gayle M. Callis HTL/HT/MT(ASCP) -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of ragh...@orchidpharma.com Sent: Tuesday, August 12, 2014 10:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Weight Loss/Weight Gain Decal _Histonet Digest, Vol 129, Issue 18 Dear all, In the weight loss/gain method how would we fix the optimal endpoint to stop the decal process based on weight gain? Will weighing omit the other ways of judgement such as needle prick, physical examination. In our tox experiments we deal with lot of femurs in one stretch and weighing method looks interesting. Pl. comment. J.Raghul Senior Research Associate Orchid Chemicals and Pharamaceuticals Limited Toxicology, Chennai,India. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: 12 August 2014 22:38 To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 129, Issue 18 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: On the lighter side... (Shirley A. Powell) 2. Bcl-2 (Heather Knight) 3. IHC Steam Strips: (Jb) 4. RE: Are Paraffin Blocks Biohazard (Michael LaFriniere) 5. RE: On the lighter side... (Mark Turner) 6. RE: On the lighter side... (Bernice Frederick) 7. Weight Loss/Weight Gain Decal (Wait, Trevor Jordan) 8. Re: Weight Loss/Weight Gain Decal (Jennifer MacDonald) 9. RE: Weight Loss/Weight Gain Decal (Wait, Trevor Jordan) 10. RE: Histonet Digest, Vol 129, Issue 17 (Mayer,Toysha N) 11. RE:On the lighter side... (Mayer,Toysha N) 12. Re: RE:On the lighter side... (Barry Rittman) 13. Re: Weight Loss/Weight Gain decalcification endpoint test (gayle callis) 14. Consult for taking abs from RUO to IVD (Patsy Ruegg) 15. RE: On the lighter side... (susan.wal...@hcahealthcare.com) 16. RE: Cytospin validation (Jamal) 17. Re: On the lighter side... (Michael Ann Jones) 18. looking for TS replacement in Histology due to retirement (Webb, Dorothy L) ---------------------------------------------------------------------- Message: 1 Date: Mon, 11 Aug 2014 13:03:38 -0400 From: "Shirley A. Powell" <powell...@mercer.edu> Subject: RE: [Histonet] On the lighter side... To: Ingles Claire <cing...@uwhealth.org>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <9BF995BC0E47744E9673A41486E24EE25BFB25FD10@MERCERMAIL.MercerU.local> Content-Type: text/plain; charset="iso-8859-1" I agree. :) -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Monday, August 11, 2014 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... Old histologists never die, they are just well fixed... Claire ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Michael Ann Jones [mjo...@metropath.com] Sent: Monday, August 11, 2014 9:07 AM To: Edwards, Richard; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] On the lighter side... 25 years, ("what?s that in micron?s??) Bernice, you are too funny!! (lots of tenure here . . .lotsa brain cells) Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 mjo...@metropath.com On 8/11/14, 5:17 AM, "Edwards, Richard" <r...@leicester.ac.uk> wrote: >Sniffed my first formalin and saw first post-mortem November 1965. > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Mon, 11 Aug 2014 13:18:57 -0400 From: Heather Knight <heather.l.knig...@gmail.com> Subject: [Histonet] Bcl-2 To: histonet@lists.utsouthwestern.edu Message-ID: <CABEU4_xVVjqyPu9o8=ONJA__mLNOXAcRmt6pK2oLcSAKp=n...@mail.gmail.com> Content-Type: text/plain; charset=UTF-8 Hi everyone- Just wondering if anyone has a working protocol for Bcl-2 in FFPE mouse tissue? If so, can you share both the protocol and the antibody information? We have tried numerous antibodies over the years with very limited success. Thank you for your help!! Best, Heather Knight ------------------------------ Message: 3 Date: Mon, 11 Aug 2014 10:18:53 -0700 From: Jb <craiga...@gmail.com> Subject: [Histonet] IHC Steam Strips: To: Histonet@lists.utsouthwestern.edu Message-ID: <375f4ef9-47b6-46cd-b5b1-b511309ef...@gmail.com> Content-Type: text/plain; charset=us-ascii Does anyone use IHC steam strips in their decloaking chamber? The question is do you run a new steam strip each run and log each individual run w/it's own steam strip? Thank you, Sent from my iPhone ------------------------------ Message: 4 Date: Mon, 11 Aug 2014 17:43:34 +0000 From: Michael LaFriniere <michael.lafrini...@ccplab.com> Subject: RE: [Histonet] Are Paraffin Blocks Biohazard To: Dawn Bugge <drbu...@gmail.com>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <4a2a16b9707ce04e9cb6c82dc18c1d296a0...@ahcmsasexch00.my.ahc.local> Content-Type: text/plain; charset="us-ascii" Dawn, The only study I know of is on CJD crutsfeldt-Jacobs Disease (know to survive formalin fixation and routine processing protocols, the CDC web site has additional information, In my laboratories I put all blocks in hazardous waste for incineration disposal. It is not that costly just to be on the safe side. Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology Laboratory 12041 Bournefield Way, Suite A * Silver Spring, MD 20904 P: 240.471.3427 * F: 240.471.3401 * Cell 410-940-8844 michael.lafrini...@ccplab.com -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dawn Bugge Sent: Friday, August 08, 2014 2:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Are Paraffin Blocks Biohazard Hello Histo World! Our pathologist for our private GI lab would like me to find out if anyone has done a study to determine if the paraffin blocks, once they have been processed, are considered biohazard. I have searched high and low and can find many people stating that the blocks are not bioharzard, with the exception of neurological tissue, but they don't state how they know this. He would like me to reference an actual study to prove that someone has actually looked into this. Any one know of something like this? I know common sense would say that once the tissues have been in formalin for hours, than on the processor for hours that the tissue would be non biohazard and completely safe. Thanks for your help :) -- Dawn R Bugge HT(ASCP), Lab Manager Seattle Histology Dawns Usborne Books Website <http://x3128.myubam.com/> _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Mon, 11 Aug 2014 17:49:13 +0000 From: Mark Turner <mtur...@csilaboratories.com> Subject: RE: [Histonet] On the lighter side... To: "Shirley A. Powell" <powell...@mercer.edu>, Ingles Claire <cing...@uwhealth.org>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <643626b74de2814d8537057f40e1a10b0cad4...@csi-mx-nodea.csi-LABS.local> Content-Type: text/plain; charset="iso-8859-1" Saves on embalming costs.... Mark Turner, Ph.D., HT(ASCP)QIHC Manager, Histology/IHC ? -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Monday, August 11, 2014 1:04 PM To: Ingles Claire; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... I agree. :) -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Monday, August 11, 2014 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... Old histologists never die, they are just well fixed... Claire ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Michael Ann Jones [mjo...@metropath.com] Sent: Monday, August 11, 2014 9:07 AM To: Edwards, Richard; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] On the lighter side... 25 years, ("what?s that in micron?s??) Bernice, you are too funny!! (lots of tenure here . . .lotsa brain cells) Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 mjo...@metropath.com On 8/11/14, 5:17 AM, "Edwards, Richard" <r...@leicester.ac.uk> wrote: >Sniffed my first formalin and saw first post-mortem November 1965. > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Mon, 11 Aug 2014 17:53:31 +0000 From: Bernice Frederick <b-freder...@northwestern.edu> Subject: RE: [Histonet] On the lighter side... To: Mark Turner <mtur...@csilaboratories.com>, "Shirley A. Powell" <powell...@mercer.edu>, Ingles Claire <cing...@uwhealth.org>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <62c639732d3f274daced033ebdf6adaf2f0f3...@evcspmbx2.ads.northwestern.edu> Content-Type: text/plain; charset="iso-8859-1" Or we'll live forever..... Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Turner Sent: Monday, August 11, 2014 12:49 PM To: Shirley A. Powell; Ingles Claire; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... Saves on embalming costs.... Mark Turner, Ph.D., HT(ASCP)QIHC Manager, Histology/IHC ? -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Monday, August 11, 2014 1:04 PM To: Ingles Claire; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... I agree. :) -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Monday, August 11, 2014 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... Old histologists never die, they are just well fixed... Claire ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Michael Ann Jones [mjo...@metropath.com] Sent: Monday, August 11, 2014 9:07 AM To: Edwards, Richard; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] On the lighter side... 25 years, ("what?s that in micron?s??) Bernice, you are too funny!! (lots of tenure here . . .lotsa brain cells) Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 mjo...@metropath.com On 8/11/14, 5:17 AM, "Edwards, Richard" <r...@leicester.ac.uk> wrote: >Sniffed my first formalin and saw first post-mortem November 1965. > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Mon, 11 Aug 2014 19:26:57 +0000 From: "Wait, Trevor Jordan" <wa...@livemail.uthscsa.edu> Subject: [Histonet] Weight Loss/Weight Gain Decal To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <1407785218488.59...@livemail.uthscsa.edu> Content-Type: text/plain; charset="iso-8859-1" Hello all! I'm currently doing some decalcification and was curious if anyone had some particular advice about the weight loss/weight gain method. I understand that when the decalcification process is complete, the tissue block will begin to increase in weight. However, I'm confused when I should record the weight for the block once they have been taken out of the EDTA solution. You see, for the times I weighed the blocks before... the weights were a little skewed because there were differing amounts of solution on the blocks while they were sitting on the balance. I just want to standardize my protocol a little more so that I can be sure the block is actually gaining weight due to the calcium loss rather than just extra solution sitting on the outside of the block. Would letting the blocks sit out of solution for about 30 minutes before being weighed help with the matter? I know that the blocks take on water once they are completely decalcified so I'm not sure how much this will affect that. Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry ------------------------------ Message: 8 Date: Mon, 11 Aug 2014 12:33:51 -0700 From: Jennifer MacDonald <jmacdon...@mtsac.edu> Subject: Re: [Histonet] Weight Loss/Weight Gain Decal To: "Wait, Trevor Jordan" <wa...@livemail.uthscsa.edu> Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu>, histonet-boun...@lists.utsouthwestern.edu Message-ID: <of5e608c00.d3abf733-on88257d31.006b4264-88257d31.006b6...@mtsac.edu> Content-Type: text/plain; charset="US-ASCII" I believe this was originally from Patsy Ruegg Decalcification End Point: Weight Loss, Weight Gain 1. Blot sample to remove excess fixative 2. Weigh bone in mg, record as beginning weight 3. Next day, rinse bone, blot and weigh bone daily, record weight. Change decalcifying solution to refresh acid OR EDTA. Return bone to fresh decalcifying solution. 4. When bone begins to GAIN weight, remove from decalcifying solution, rinse and process. YOU MUST WEIGH IN MILLIGRAMS FOR ACCURACY Once calcium is totally removed (bone loses weight as this happens), water replaces the calcium and weight begins to go up. This is the point at which calcium should be totally gone. The original method used a chemical test at the end to insure no calcium was in the decalcifying solution. If you do this, you cannot stir the solution during decalcification. Be sure to suspend bone in the solution to insure all sides of bone are in contact with decalcification solution. From: "Wait, Trevor Jordan" <wa...@livemail.uthscsa.edu> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Date: 08/11/2014 12:29 PM Subject: [Histonet] Weight Loss/Weight Gain Decal Sent by: histonet-boun...@lists.utsouthwestern.edu Hello all! I'm currently doing some decalcification and was curious if anyone had some particular advice about the weight loss/weight gain method. I understand that when the decalcification process is complete, the tissue block will begin to increase in weight. However, I'm confused when I should record the weight for the block once they have been taken out of the EDTA solution. You see, for the times I weighed the blocks before... the weights were a little skewed because there were differing amounts of solution on the blocks while they were sitting on the balance. I just want to standardize my protocol a little more so that I can be sure the block is actually gaining weight due to the calcium loss rather than just extra solution sitting on the outside of the block. Would letting the blocks sit out of solution for about 30 minutes before being weighed help with the matter? I know that the blocks take on water once they are completely decalcified so I'm not sure how much this will affect that. Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Mon, 11 Aug 2014 19:35:29 +0000 From: "Wait, Trevor Jordan" <wa...@livemail.uthscsa.edu> Subject: RE: [Histonet] Weight Loss/Weight Gain Decal To: Jennifer MacDonald <jmacdon...@mtsac.edu> Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu>, "histonet-boun...@lists.utsouthwestern.edu" <histonet-boun...@lists.utsouthwestern.edu> Message-ID: <1407785730771.24...@livemail.uthscsa.edu> Content-Type: text/plain; charset="iso-8859-1" Ha Wow...that's almost too easy. Thank you for this! Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry ________________________________ From: Jennifer MacDonald <jmacdon...@mtsac.edu> Sent: Monday, August 11, 2014 2:33 PM To: Wait, Trevor Jordan Cc: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Subject: Re: [Histonet] Weight Loss/Weight Gain Decal I believe this was originally from Patsy Ruegg Decalcification End Point: Weight Loss, Weight Gain 1. Blot sample to remove excess fixative 2. Weigh bone in mg, record as beginning weight 3. Next day, rinse bone, blot and weigh bone daily, record weight. Change decalcifying solution to refresh acid OR EDTA. Return bone to fresh decalcifying solution. 4. When bone begins to GAIN weight, remove from decalcifying solution, rinse and process. YOU MUST WEIGH IN MILLIGRAMS FOR ACCURACY Once calcium is totally removed (bone loses weight as this happens), water replaces the calcium and weight begins to go up. This is the point at which calcium should be totally gone. The original method used a chemical test at the end to insure no calcium was in the decalcifying solution. If you do this, you cannot stir the solution during decalcification. Be sure to suspend bone in the solution to insure all sides of bone are in contact with decalcification solution. From: "Wait, Trevor Jordan" <wa...@livemail.uthscsa.edu> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Date: 08/11/2014 12:29 PM Subject: [Histonet] Weight Loss/Weight Gain Decal Sent by: histonet-boun...@lists.utsouthwestern.edu ________________________________ Hello all! I'm currently doing some decalcification and was curious if anyone had some particular advice about the weight loss/weight gain method. I understand that when the decalcification process is complete, the tissue block will begin to increase in weight. However, I'm confused when I should record the weight for the block once they have been taken out of the EDTA solution. You see, for the times I weighed the blocks before... the weights were a little skewed because there were differing amounts of solution on the blocks while they were sitting on the balance. I just want to standardize my protocol a little more so that I can be sure the block is actually gaining weight due to the calcium loss rather than just extra solution sitting on the outside of the block. Would letting the blocks sit out of solution for about 30 minutes before being weighed help with the matter? I know that the blocks take on water once they are completely decalcified so I'm not sure how much this will affect that. Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Mon, 11 Aug 2014 19:45:01 +0000 From: "Mayer,Toysha N" <tnma...@mdanderson.org> Subject: [Histonet] RE: Histonet Digest, Vol 129, Issue 17 To: "'histonet@lists.utsouthwestern.edu'" <histonet@lists.utsouthwestern.edu> Message-ID: <47e9b2c01dddd94881eacd2dc44ebc881b438...@d1pwpexmbx05.mdanderson.edu> Content-Type: text/plain; charset="us-ascii" Egads, I feel like a baby! 20 years registered, 3 yrs unregistered. By the way, I did see a former colleague with her ASCP certificate in her office, and the oldest sticker is from the year I was born. Sincerely, Dr.Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 Message: 2 Date: Sun, 10 Aug 2014 21:50:58 +0000 From: Sebree Linda A <lseb...@uwhealth.org> Subject: RE: [Histonet] On the lighter side... To: Jamal <j.rowa...@alborglaboratories.com>, 'Vincent Rivera' <vriv...@westderm.com>, 'Douglas Porter' <doug.por...@caplab.org>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <77dd817201982748bc67d7960f2f76af0c7...@uwhc-mbx12.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="iso-8859-1" 38 years and looking forward to retirement in a few years. Linda A. Sebree ------------------------------ Message: 11 Date: Mon, 11 Aug 2014 19:52:19 +0000 From: "Mayer,Toysha N" <tnma...@mdanderson.org> Subject: [Histonet] RE:On the lighter side... To: "'histonet@lists.utsouthwestern.edu'" <histonet@lists.utsouthwestern.edu> Message-ID: <47e9b2c01dddd94881eacd2dc44ebc881b438...@d1pwpexmbx05.mdanderson.edu> Content-Type: text/plain; charset="us-ascii" Tim, Just saw your post about the 'marketable skill'. Funny those were my mother's exact words while I was in college. She didn't care what I majored in, as long as I got a marketable skill along the way. The best advice I've ever gotten. Thanks Maria!!! (my mother) Sincerely, Dr.Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: 08 August 2014 19:26 To: 'Douglas Porter'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... Wow, I feel like a newbie! 28 years, registered. HT 13663, 1988, HTL 1369, 1992. Electron Microscopy Technologist, #604, 1982. Like most, I never heard of "histology" until I walked into a hospital lab on my first day as an EM tech. I had seen slides made in college, but no one ever mentioned it could be an actual profession. I was more taken with the electron microscope, and there was (is) a 2-year program at the community college in the town I grew up in (Delta College, Stockton, CA). So AFTER getting a BA in Zoology, I went there to get a marketable skill. At that time EM was still used for tumor dx, so when I started it was about half tumor, half kidney. I was lucky enough to get involved in histology and set up the IHC lab at the small community hospital I worked at (as an EM tech) and so ended up phasing myself almost out of an EM job. The IHC took over all the tumor dx from EM. Later I left EM altogether and did IHC exclusively for 15 years. But, like most, I learned Histotechnology on the job but was lucky enough to work for a pathologist who believed in developing his techs - to the point of paying for meetings out of his own pocket. Only now do I know how fortunate I was to work for someone like that. Because of him we had developed a culture in the small histo lab (4 men!!) of learning. We studied together one night a week for the HT exam and all passed (and the practical!). Again, that was a fortunate experience, not very often seen in labs. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA ------------------------------ Message: 12 Date: Mon, 11 Aug 2014 15:45:05 -0500 From: Barry Rittman <barryritt...@gmail.com> Subject: Re: [Histonet] RE:On the lighter side... To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <ca+0tsf1qrppfye2xsq+kt-8kx_fgd38fvr657fyrzby-4es...@mail.gmail.com> Content-Type: text/plain; charset=UTF-8 Well fixed in the histological sense I hope. Barry On Mon, Aug 11, 2014 at 2:52 PM, Mayer,Toysha N <tnma...@mdanderson.org> wrote: > Tim, > > Just saw your post about the 'marketable skill'. Funny those were my > mother's exact words while I was in college. She didn't care what I > majored in, as long as I got a marketable skill along the way. > The best advice I've ever gotten. > Thanks Maria!!! (my mother) > > Sincerely, > > Dr.Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education > Coordinator Program in Histotechnology School of Health Professions UT > M.D. Anderson Cancer Center > 713.563-3481 > > > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu [mailto: > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy > Sent: 08 August 2014 19:26 > To: 'Douglas Porter'; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] On the lighter side... > > Wow, I feel like a newbie! 28 years, registered. HT 13663, 1988, HTL 1369, > 1992. Electron Microscopy Technologist, #604, 1982. > > Like most, I never heard of "histology" until I walked into a hospital lab > on my first day as an EM tech. I had seen slides made in college, but no > one ever mentioned it could be an actual profession. I was more taken with > the electron microscope, and there was (is) a 2-year program at the > community college in the town I grew up in (Delta College, Stockton, CA). > So AFTER getting a BA in Zoology, I went there to get a marketable skill. > At that time EM was still used for tumor dx, so when I started it was about > half tumor, half kidney. I was lucky enough to get involved in histology > and set up the IHC lab at the small community hospital I worked at (as an > EM tech) and so ended up phasing myself almost out of an EM job. The IHC > took over all the tumor dx from EM. Later I left EM altogether and did IHC > exclusively for 15 years. But, like most, I learned Histotechnology on the > job but was lucky enough to work for a pathologist who believed in > developing his techs - to the point of paying for meetings out of his own > pocket. Only now do I know how fortunate I was to work for someone like > that. Because of him we had developed a culture in the small histo lab (4 > men!!) of learning. We studied together one night a week for the HT exam > and all passed (and the practical!). Again, that was a fortunate > experience, not very often seen in labs. > > Tim Morken > Supervisor, Histology, Electron Microscopy and Neuromuscular Special > Studies UC San Francisco Medical Center San Francisco, CA > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 13 Date: Mon, 11 Aug 2014 15:35:09 -0600 From: "gayle callis" <gayle.cal...@bresnan.net> Subject: [Histonet] Re: Weight Loss/Weight Gain decalcification endpoint test To: <histonet@lists.utsouthwestern.edu> Message-ID: <000601cfb5ac$22447d70$66cd7850$@bresnan.net> Content-Type: text/plain; charset="us-ascii" Trevor and Jennifer, This method originally came from Mawhinney et al. Control of rapid nitric acid decalcification J Clin Pathol 1984 37:1409-1415 and was cited by Cathy Sanderson (Mayton)in a publication using EDTA, found in Biotechnic and Histochemistry. Mawhinney acutally did a chemical test on final acid change to see that calcium was not present, but we never had to do that. If you end up with a bit of residual calcium in block, I would surface decalcify at microtomy. I used it for years when we downsized and gave away our FAXITRON. Radiography is still the most accurate, and if you had either a micro CT or digital FAXITRON available, it would be a better test. I used Cathy's method and will be happy to send the original publication for anyone's files/future referencing. A chemical test for EDTA is a pain to do if a FAXITRON is not available. I have put the full method below, with a bit more detail. WEIGHT LOSS/WEIGHT GAIN ENDPOINT TEST This is a method of choice for EDTA decalcification although it was originally used by Mawhinney et al for testing nitric acid decalcification. Many samples can be decalcified together in one container, i.e. 25 mouse femurs in 1 liter of 10% formic acid. If all the samples are the same size, i.e. mouse femurs, tibia, paws, choose several as representative samples and test only those to save time. Always suspend bones in the decalcifier. Requires a balance that reads in milligrams to 3 places for greatest accuracy. Specimen must be blotted free of fluid for accurate weighing each time you weigh the sample. We suspend bones in nylon specimen bags for easy removal to weigh. Bags can be marked with pencil too. Technique: 1. Rinse NBF off bone, blot with paper towel, WEIGH BONE, RECORD BEGINNING WEIGHT. Suspend bone in acid or EDTA decalcifier. During acid decalcification CO2 bubbles are given off, so stir during decalcification to release bubbles or small samples will float. EDTA does not create CO2 bubbles, only acids. Large bones can be started at end of day in acid decalcifier and sit overnight with testing the next morning. 2. After 4 to 5 hours in acid or overnight in EDTA, remove bone, rinse with water, BLOT, weigh. RECORD WEIGHT. If bone shows loss of weight, change acid decalcifier to refresh acid. Return samples to resume decalcification, and repeat as many times as necessary. EDTA should be changed but not as often as acid. Always use a large volume of decalcifier i.e. 20:1 or more. Remove bone from specimen bag, and place in weighing boat to protect balance from acids/EDTA. 3. When bone begins to GAIN WEIGHT, the bone is decalcified. Once calcium is removed, water is taken on and the weight increases. This water does not affect the bone. 4. Rinse bone with running tap water for an hour or longer to remove these decalcifiers. Either store in 70% alcohol or process. Store endpoint tested decalcified bones in 70% alcohol while waiting for other samples to finish decalcifying and mass processing run. Reminders: For EDTA, one can suspend bones and check every day for accuracy but bones can be left in the EDTA over a weekend or several days without damage as long as the bones were well fixed. Acid decalcified bones cannot be left over a weekend, remove from acid, put in NBF to stop decalcification. Bones should be endpoint tested before stopping decalcification so you can resume decalcification on the next working day. Rinse off NBF briefly before resuming decalcification. Do not overexpose bones to acids or you will damage antigens and nuclear staining. Enjoy the method, as it truly is fast and easy. Gayle M. Callis HTL/HT/MT(ASCP) ____________________________________________________________________________ _____ Ha Wow...that's almost too easy. Thank you for this! Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry ________________________________ From: Jennifer MacDonald < <http://lists.utsouthwestern.edu/mailman/listinfo/histonet> JMacDonald <@t> mtsac.edu> Sent: Monday, August 11, 2014 2:33 PM To: Wait, Trevor Jordan Cc: <http://lists.utsouthwestern.edu/mailman/listinfo/histonet> histonet <@t> lists.utsouthwestern.edu; <http://lists.utsouthwestern.edu/mailman/listinfo/histonet> histonet-bounces <@t> lists.utsouthwestern.edu Subject: Re: [Histonet] Weight Loss/Weight Gain Decal I believe this was originally from Patsy Ruegg Decalcification End Point: Weight Loss, Weight Gain 1. Blot sample to remove excess fixative 2. Weigh bone in mg, record as beginning weight 3. Next day, rinse bone, blot and weigh bone daily, record weight. Change decalcifying solution to refresh acid OR EDTA. Return bone to fresh decalcifying solution. 4. When bone begins to GAIN weight, remove from decalcifying solution, rinse and process. YOU MUST WEIGH IN MILLIGRAMS FOR ACCURACY Once calcium is totally removed (bone loses weight as this happens), water replaces the calcium and weight begins to go up. This is the point at which calcium should be totally gone. The original method used a chemical test at the end to insure no calcium was in the decalcifying solution. If you do this, you cannot stir the solution during decalcification. Be sure to suspend bone in the solution to insure all sides of bone are in contact with decalcification solution. From: "Wait, Trevor Jordan" < <http://lists.utsouthwestern.edu/mailman/listinfo/histonet> WaitT <@t> livemail.uthscsa.edu> To: " <http://lists.utsouthwestern.edu/mailman/listinfo/histonet> histonet <@t> lists.utsouthwestern.edu" < <http://lists.utsouthwestern.edu/mailman/listinfo/histonet> histonet <@t> lists.utsouthwestern.edu> Date: 08/11/2014 12:29 PM Subject: [Histonet] Weight Loss/Weight Gain Decal Sent by: <http://lists.utsouthwestern.edu/mailman/listinfo/histonet> histonet-bounces <@t> lists.utsouthwestern.edu ________________________________ Hello all! I'm currently doing some decalcification and was curious if anyone had some particular advice about the weight loss/weight gain method. I understand that when the decalcification process is complete, the tissue block will begin to increase in weight. However, I'm confused when I should record the weight for the block once they have been taken out of the EDTA solution. You see, for the times I weighed the blocks before... the weights were a little skewed because there were differing amounts of solution on the blocks while they were sitting on the balance. I just want to standardize my protocol a little more so that I can be sure the block is actually gaining weight due to the calcium loss rather than just extra solution sitting on the outside of the block. Would letting the blocks sit out of solution for about 30 minutes before being weighed help with the matter? I know that the blocks take on water once they are completely decalcified so I'm not sure how much this will affect that. Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry _______________________________________________ Histonet mailing list <http://lists.utsouthwestern.edu/mailman/listinfo/histonet> Histonet <@t> lists.utsouthwestern.edu <http://lists.utsouthwestern.edu/mailman/listinfo/histonet> http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Mon, 11 Aug 2014 17:04:32 -0600 From: Patsy Ruegg <prueg...@hotmail.com> Subject: [Histonet] Consult for taking abs from RUO to IVD To: "Histonet@Lists. Edu" <histonet@lists.utsouthwestern.edu> Message-ID: <bay178-w34a801440a69fa187c6061d8...@phx.gbl> Content-Type: text/plain; charset="iso-8859-1" Colleagues, I am consulting with an antibody vendor who needs a consultant to help them take their abs from RUO to IVD. If you know anyone who does this type of work you could recommend would you please have them contact me directly and I will pass their contact info on. Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com pru...@ihctech.net ------------------------------ Message: 15 Date: Tue, 12 Aug 2014 01:58:14 -0500 From: <susan.wal...@hcahealthcare.com> Subject: RE: [Histonet] On the lighter side... To: <cing...@uwhealth.org>, <histonet@lists.utsouthwestern.edu> Message-ID: <4bf03f5404ebde409af9232da74b9ded2fbffc4...@fwdcwpmsgcms09.hca.corpad.net> Content-Type: text/plain; charset="iso-8859-1" Also heard" Histotechs are good embed!!!" LOL -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Monday, August 11, 2014 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... Old histologists never die, they are just well fixed... Claire ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Michael Ann Jones [mjo...@metropath.com] Sent: Monday, August 11, 2014 9:07 AM To: Edwards, Richard; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] On the lighter side... 25 years, ("what?s that in micron?s??) Bernice, you are too funny!! (lots of tenure here . . .lotsa brain cells) Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 mjo...@metropath.com On 8/11/14, 5:17 AM, "Edwards, Richard" <r...@leicester.ac.uk> wrote: >Sniffed my first formalin and saw first post-mortem November 1965. > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Tue, 12 Aug 2014 10:49:24 +0300 From: "Jamal" <j.rowa...@alborglaboratories.com> Subject: [Histonet] RE: Cytospin validation To: <histonet@lists.utsouthwestern.edu> Message-ID: <013e01cfb601$f0da61e0$d28f25a0$@rowa...@alborglaboratories.com> Content-Type: text/plain; charset="us-ascii" Good day colleagues Ignoring my previous message what it means: no one did validation for Cytospin !! or no one want to share his information ?? or no one received my message !!?? Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| <mailto:j.rowa...@alborglaboratories.com> j.rowa...@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | <http://www.alborglaboratories.com/> www.alborglaboratories.com From: Jamal [mailto:j.rowa...@alborglaboratories.com] Sent: Monday, August 11, 2014 1:47 PM To: 'histonet@lists.utsouthwestern.edu' Subject: Cytospin validation Hi I need to do validation for my Shandon Cytospin 4, can anyone send me the validation procedure and form. Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| <mailto:j.rowa...@alborglaboratories.com> j.rowa...@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | <http://www.alborglaboratories.com/> www.alborglaboratories.com ------------------------------ Message: 17 Date: Tue, 12 Aug 2014 13:00:08 +0000 From: Michael Ann Jones <mjo...@metropath.com> Subject: Re: [Histonet] On the lighter side... To: "susan.wal...@hcahealthcare.com" <susan.wal...@hcahealthcare.com>, "cing...@uwhealth.org" <cing...@uwhealth.org>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <d00f6be1.2712%mjo...@metropath.com> Content-Type: text/plain; charset="euc-kr" Ha ha!! Good one - Michael Ann On 8/12/14, 12:58 AM, "susan.wal...@hcahealthcare.com" <susan.wal...@hcahealthcare.com> wrote: >Also heard" Histotechs are good embed!!!" LOL > >-----Original Message----- >From: histonet-boun...@lists.utsouthwestern.edu >[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ingles >Claire >Sent: Monday, August 11, 2014 12:00 PM >To: histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] On the lighter side... > > >Old histologists never die, they are just well fixed... >Claire >________________________________________ >From: histonet-boun...@lists.utsouthwestern.edu >[histonet-boun...@lists.utsouthwestern.edu] on behalf of Michael Ann >Jones [mjo...@metropath.com] >Sent: Monday, August 11, 2014 9:07 AM >To: Edwards, Richard; histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] On the lighter side... > >25 years, ("what??s that in micron??s???) Bernice, you are too funny!! >(lots of tenure here . . .lotsa brain cells) Michael Ann Jones, HT (ASCP) >Histology Manager Metropath >7444 W. Alaska Dr. #250 >Lakewood, CO 80226 >303.634.2511 >mjo...@metropath.com > > > > >On 8/11/14, 5:17 AM, "Edwards, Richard" <r...@leicester.ac.uk> wrote: > >>Sniffed my first formalin and saw first post-mortem November 1965. >> >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 18 Date: Tue, 12 Aug 2014 11:18:12 -0500 From: "Webb, Dorothy L" <dorothy.l.w...@healthpartners.com> Subject: [Histonet] looking for TS replacement in Histology due to retirement To: "'histonet@lists.utsouthwestern.edu'" <histonet@lists.utsouthwestern.edu> Message-ID: <65365f35c0f2ef4d846ec3ca73e49c4302f6d319b...@hpemx3.healthpartners.int> Content-Type: text/plain; charset="us-ascii" Histology Technical Specialist Position in St. Paul, MN Regions Hospital is a Level I Adult and Pediatric trauma Center and teaching hospital serving Minnesota and western Wisconsin for more than 130 years. Regions is a private, non-profit hospital providing outstanding care in women's health, heart, cancer, surgery, orthopaedics, neurosciences, burn, emergency care, and more. Regions Hospital Lab is a state of the art. Regions is a part of the HealthPartners Family of Care. Additional information is available at regionshospital.com Regions Hospital employees enjoy opportunities for personal and professional growth available only at one of the top teaching hospitals in the Twin Cities area. Our dedication to patient care and commitment to a healthy workplace, and "Best Care, Best Experience", has allowed us to be recognized by the Minnesota Hospital Association with the Best Minnesota Hospital Workplace Award. This position will oversee the technical component of a work team in the histology laboratory including; implementation of methodologies, supply ordering and inventory management, interpretation of laboratory results for physicians and other hospital staff; performance of laboratory tests in the section assigned; assisting in performance evaluations; assisting in supervision of testing personnel; development and implementation of training and competency programs for histotechnicians and histology students interns, and to perform related duties as assigned. Qualifications: Graduation from an accredited college or university with an Associates (AA) or bachelor's degree in medical technology, histology, biology or related field; plus specialized training in laboratory science. A master's degree in related field may substitute for the above educational and registration requirements. Bachelor's degree preferred. At least four (4) years of experience as a Histotechnician or Histotechnologist or related field with at least two (2) years of experience in the specialty occurring within the last four years. How to Apply Apply online at www.regionshospital.com<https://careers.peopleclick.com/careerscp/client_hea lthpartners/regions_external/jobDetails.do?functionName=getJobDetail&jobPost Id=59124&localeCode=en-us> Additional Information We are an Equal Opportunity Employer and do not discriminate against applicants due to race, ethnicity, gender, veteran status, or on the basis of disability or any other federal, state or local protected class. ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. 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