Hi Everyone, 

I typically used charged slides to mount my 50 um floating brain sections. 
However, these seem pricey and I remember subbing slides in the past. I most do 
DAB staining, native fluorescence and occasionally immunofluorescence. Can 
someone tell me a good and easy protocol for cleaning and subbing slides? 

I currently have relatively old gelatin (7+ years?), Type B 225 bloom. Is this 
sufficient? A lot of protocols call for type A.

Thanks!

Caroline Bass
University at Buffalo
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