For those of you who quantify optical densities of DAB, IP, etc.; and for those who measure for areas, counts, etc (morphometry); please read below regarding a free webinar. Note that the webinar will present a new product and software solution. I encourage you to listen, even if you are not in a position to purchase this product/software solution, because you will learn about linearity, the importance of linear tones when performing optical density measurements, and the possibility that your camera system is not producing linear images and is, therefore, providing an image that contains erroneous data. Morphometry and Density/Intensity Readings: Avoid Errors in Quantification ------------------------------ Tuesday, October 21, 2014 1:00 PM - 1:30 PM EDT Webinar Registration: https://www2.gotomeeting.com/register/945357514
Introduction by Michael Linden, MD, PhD (Assistant Professor, Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, MN) Presenter: Jerry Sedgewick (Imaging and Analysis, LLC; author, consultant and recognized authority in post-processing, analysis and quantification of scientific images) If you have done quantitation, you know that inconsistent images require subjective means for setting thresholds when segmenting objects for measurement. You need an objective method to set thresholds for measurement of area, length, count, etc., and be able to apply that method across multiple images. When quantifying densities and intensities, it is imperative that images contain equal increments of grey or color values across the dynamic range (image linearity). Can you trust your images? A recent survey found that approximately 50% of color camera systems offer software settings that can result in non-linear color images. If you’ve been quantifying immunostained cells or cellular structures (i.e. DAB, BCIP/NBT, X-Gal, etc.), you may have presented false data. This webinar explores quantitative methods that employ a single threshold for any number of color brightfield images. You will learn how to create consistent images, without subjective adjustments, for subsequent quantization. You will learn how to confirm your imaging system delivers scientific images that are linear. You will also learn how to correct images taken over a time course while keeping tones and colors linear. -- Jerry (Gerald) Sedgewick quickphotoshop.com http://www.imagingandanalysis.com Author: "Scientific Imaging with Photoshop: Methods, Measurement and Output" Automated Image Measurement services and systems; On-Site and on-line training for image acquisition/processing/quantitation in science, microscopy and medicine. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet