You can try to fill the TMA through the cassette from the bottom.  Place the 
TMA in a mold that fits the TMA, heat it for about 2 min and then try filling 
the TMA from the cassette side.  You can also dip the heated TMA block in a 
paraffin bath to fill the hole or use a plastic pipette to fill the gap.  
Anyway you get the paraffin to fill the hole you will need to melt the whole 
TMA for a bit to create a uniform block.  If you have more questions give me a 
call.

Aprill Watanabe, B.S.
Laboratory Coordinator
Macromolecular Analyte Processing Center (MAPC)
Integrated Cancer Genomics Division
Translational Genomics Research Institute (TGen)
445 North 5th Street
Phoenix, AZ 85004
Office: 602-343-8822
Fax: 602-343-8840
Cell: 602-481-8654
email: awatan...@tgen.org
website: www.tgen.org
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