Neelam, If you are referring to immunolabelling for transmission electron microscopy, it is relatively simple to apply and this worked wonderfully for projects we did, to use 2 antibodies to two different epitopes but each antibody with a different size gold particle attached. The gold particles are between a few nanometers up to 30 or 40 nanometers in diameter which are attached to antibodies and so are easy to tell apart from one another. Ray Lake Forest Park, WA
----- Original Message ----- From: "Neelam Joshi" <neelam.jo...@nephropath.com> To: histonet@lists.utsouthwestern.edu Sent: Thursday, October 23, 2014 5:42:21 AM Subject: [Histonet] Double Labeling EM Blocks Hello, Does anyone has a procedure for double labeling EM Blocks with two identifier? Please let me know as soon as possible. Thanks you all! Neelam Joshi,HT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
