Tiffany, Have used 10%NBF on muscles but also alcoholic fixatives -alcoholic formalin or absolute- just always preferred 10%NBF since it gave the morphology and counterstaining I wanted. diastase in a 6.0pH buffer (don't heat above 40 degrees if trying to speed up heating-kill the diastase) and always stayed away from di water on frozen sections. di water too variable and fickle. Ray Koelling Lake Forest Park
----- Original Message ----- From: "Tiffany Passaro" <tpass...@cellnetix.com> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Sent: Monday, January 5, 2015 3:22:32 PM Subject: [Histonet] PASD muscle stains Greetings, I am looking for fixatives that others are using in their labs for the PASD stain on fresh frozen muscle tissue. Currently we are fixing in 10% NBF. Thanks in advance for any info on this. Tiffany DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
