I also noticed stain fading with time - controls cut way ahead of staining? Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 mjo...@metropath.com
On 1/23/15, 1:00 PM, "Wheelock, Timothy R." <twheel...@mclean.harvard.edu> wrote: >Hi Jeffery: > >Yes, I notice the same "paraffin dust bunnies", perhaps especially since >I use a embedding paraffin that has a fair amount of plastic. >Before I embed my brain tissue, I mix the embedding media thoroughly, >until the solution is clear. > >As for your Congo Red problem, I wish that I could help you. >I am now experiencing the same problem, except that it affects the >positive controls as well. >The staining is there, but much fainter than it should be. > >I usually immerse albumin coated slides in 1% Congo red for 15 minutes, >differentiate them with Potassium Hydroxide for 3 dips, counterstain with >Harris Hematoxylin for 1 minute, differentiate with acid alcohol for 5-10 >dips, immerse in running tap water for 10 minutes, immerse in 95% ethanol >for 2 dips, then dehydrate and mount. > >Tim > > > > >The information in this e-mail is intended only for the person to whom it >is >addressed. If you believe this e-mail was sent to you in error and the >e-mail >contains patient information, please contact the Partners Compliance >HelpLine at >http://www.partners.org/complianceline . If the e-mail was sent to you in >error >but does not contain patient information, please contact the sender and >properly >dispose of the e-mail. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet