Thanks everybody for the ideas and the expression of sympathy! Our conclusion is that it's not possible to soften up a hard fruit pit for paraffin sectioning in a standard Histology lab. We never tried celloidin embedding, so that's still a possiblility.
The best approach might be a bone or mineral grinder that can produce microscopic sections of hard material. Thanks again, Hanna Preiszner ETSU/QCOM ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Preiszner, Johanna [preis...@mail.etsu.edu] Sent: Tuesday, February 03, 2015 4:20 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] peach stone (pit) sectioning? Hi Netters, does anybody know how I could soften up a hard fruit pit for sectioning? Thanks, Hanna Preiszner ETSU/QCOM _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
