Hi there,
we are having problems trying to cut some embedded samples (they crumble
in the bath and the few cuts we manage to get into HE are crap). These
are formalin fixed samples (bovine foetal and placenta samples) which
went straight from formlin into 100º ethanol for the dehydration before
clearing. I was wondering if such drastic dehydration step (no 60º, 70º
or 90º ethanol before the 100º) could have damage the tissue. Has anyone
have a similar issue before? do you think the samples are ruined for
histology?
Thanks a lot for your help
Regards
Julio
Instituto de Ganaderia de Montaña
Spain
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